Question

1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a solution of pigment X that shows an absorbance of 0.443 at 370nm? (Assume linearity) b. If you have 10 ml of this solution, how many ug of pigment X do you have? 5. Sample calculations Show your calculations. a. How would you prepare 1. Oml of a 0.6mg/ml protein sample from a 1.Omg/ml protein stock solution in a given phosphate buffer? b. 1 ml of water and 1.0 ml of a solution "X" mixed together is a 1:2 dilution of 'X' in water.

Answer 1

solution :- 1 % V/v means = volume of product X 100 - Total volume of solvent Given Total volume = 125ml • v/v - 10 ) 10 = volume of product X 100 125 ml J volume of product (acetic acid) = 10 X 1925 ml ' = 11250ml so to prepare 125 ml 1215 me of autic acid is needed to be add in Ho. UDU