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SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
Part A. caculate the molarity. 6.65 g of NaCl in 250. mL of a NaCl solution Part B. calculate the volume, in liters, f...
Part A. caculate the molarity. 6.65 g of NaCl in 250. mL of a NaCl solution Part B. calculate the volume, in liters, for each of the following 34.0 g of NaHCO3 from a 1.00 M NaHCO3 solution (express your answer as three significant figures) Part C. calculate the final concentration of each of the following 1. a 20.0 ml sample of an 7.0% (m/v) KBr solution is diluted with water so that the final volume is 222.0 mL 2....
You are given a stock solution of ribosylated collagen. In order to complete your experiment, you...
You are given a stock solution of ribosylated collagen. In order to complete your experiment, you will need four 1ml aliquots of ribosylated collagen in phosphate buffered saline (1M), each containing 67.5 ng/ml. The stock concentration is 10 ug/ml, What steps are required to achieve the required concentrations? During lab a classmate accidentally breaks an Erlenmeyer flask: a single shard causes a 50mm laceration between his elbow and wrist. You decide that the best way to keep the wound clean...
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20
1. You prepare a NaCl solution by dissolving making 250mL solution out of 0.1409 g of...
1. You prepare a NaCl solution by dissolving making 250mL solution out of 0.1409 g of NaCl (MW = 58.443 g/mol). You use this solution to standardize a Hg(NO3)2 solution. The volume of Hg(NO3)2 needed to titrate 10 mL of NaCl is 4.017 mL what is the molarity of the a Hg(NO3)2 solution? Note: Hg2+ + 2 Cl → HgCl2 2. Using the Folin-Wu tungstic acid method you pipet 10 mL of a serum sample and precipitate the protein (Note:...
how many millimeters of a 90.0% (w/v) NaCl solution will you need to prepare 500mL of...
how many millimeters of a 90.0% (w/v) NaCl solution will you need to prepare 500mL of a 0.225% NaCl solution? What amount of NaCl (MW 58.44) in grand must be combined with water to prepare 1000mL of a 0.90% (w/v) normal saline solution? What amount of NaHCO3 (MW 88.01) in grams is needed to prepare 5000mL of 0.024 M solution? Please explain - thank you!
1. A series of dilute NaCl solutions are prepared starting with an initial stock solution of...
1. A series of dilute NaCl solutions are prepared starting with an initial stock solution of 0.100 M NaCl. Solution A is prepared by pipeting 10 mL of the stock solution into a 250-mL volumetric flask and diluting to volume. Solution B is prepared by pipeting 25 mL of solution A into a 100-mL volumetric flask and diluting to volume. Solution C is prepared by pipeting 20 mL of solution B into a 500-mL volumetric flask and diluting to volume....
17. What volume of 0.345 M NaCl solution is needed to obtain 10.0 g of NaCl?...
17. What volume of 0.345 M NaCl solution is needed to obtain 10.0 g of NaCl? 18 How many milliliters of a 0.0015 M cocaine hydrochloride (C17H22ClNO4) solution is needed to obtain 0.010 g of the solute? 19.. A solution having an initial concentration of 0.445 M and the initial volume of 45.0 mL is diluted to 100.0 mL. What is its final concentration? 20. A 50.0 mL sample of salt water that is 3.0% m/v is diluted to 950...
A 2.550×10−2M solution of NaCl in water is at 20.0∘C. The sample was created by dissolving...
A 2.550×10−2M solution of NaCl in water is at 20.0∘C. The sample was created by dissolving a sample of NaCl in water and then bringing the volume up to 1.000 L. It was determined that the volume of water needed to do this was 999.3 mL . The density of water at 20.0∘C is 0.9982 g/mL. A) Calculate the molality of the salt solution. B) Calculate the mole fraction of salt in this solution. C) Calculate the concentration of the...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
You are given a stock solution of ribosylated collagen. In order to complete your experiment, you will need four 1ml aliquots of ribosylated collagen in phosphate buffered saline (1M), each containing 67.5 ng/ml. The stock concentration is 10 ug/ml, What steps are required to achieve the required concentrations? During lab a classmate accidentally breaks an Erlenmeyer flask: a single shard causes a 50mm laceration between his elbow and wrist. You decide that the best way to keep the wound clean...
1. You prepare a NaCl solution by dissolving making 250mL solution out of 0.1409 g of NaCl (MW = 58.443 g/mol). You use this solution to standardize a Hg(NO3)2 solution. The volume of Hg(NO3)2 needed to titrate 10 mL of NaCl is 4.017 mL what is the molarity of the a Hg(NO3)2 solution? Note: Hg2+ + 2 Cl → HgCl2 2. Using the Folin-Wu tungstic acid method you pipet 10 mL of a serum sample and precipitate the protein (Note:...
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