You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
- You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.
|
Sample |
Protein Concentration mg/mL |
Protein Sample (microliter) |
Water (microliter) |
4X Sample Buffer (microliter) |
Total Sample Volume (microliter) |
|
Total Lysate (-) |
1 mg/mL |
20 |
|||
|
E1 |
0.07 mg/mL |
20 |
|||
|
E2 |
0.15 mg/mL |
20 |
|||
|
E3 |
0.2 mg/mL |
20 |
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You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
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a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
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SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh out. 1 (a). You need 500mL of 15% (w/v) NaCl 1(b) What approximate volume of water would you dissolve the NaCl in? Please give a range of volumes. 1(c). Assuming that adding the NaCI to your original volume of water caused the volume to increase by 50mL before you added additional water to reach your final volume, what is the DENSITY of your final...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
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