Question

4a) Describe how you would prepare a 1-liter aqueous solution of each of the following reagents: 1M glycine

5) Why is deionized (ion-exchange) water not suitable as a solvent for use in UV absorbance measurements?

7) If you mix 1mL of the 1M glycine solution in problem 4a with 9ml of water, what is the final concentration of this diluted solution in mM?

9) You have just prepared a solution by weighing 20g of sucrose, transferring it to a 10liter volumetric flask, and adding water to the line. Calculate the concentration of the sucrose solution in terms of mM, mg/ml and %(wt/vol).

Answer 1

​​​​​​Molarity = (mass required/molar mass) × (1/volume in litres)

Molarity = 1 M

Molar mass of glycine = 75 g per mol

Volume = 1 L

Required mass = ? = x

1 = (x/75) × (1/1)

x = 75 g

To make 1 M, 1 L of glycine, we need to dissolve 75 gram of glycine in 1 L of solution.

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