Question

You are given a 100 mg/ml protein solution, how will you prepare 20 μL of a 70 mg/ml protein solution?

Answer 1

Answer:

Dear Student,

Here we are going to use the relative expressions between concentrations and volumes.

Cini.Vini = C_fin.V_fin

Here the initial concentration given (C_ini)= 100 mg/mL

Final concentration (C_fin) = 70mg/mL

and final volume = 20uL

The initial volume of solution required = "C" uL

Hence, V_ini = (70 mg/mL X 20 uL)/100 mg/mL = 14 uL

Hence, 14 uL of protein solution (with 100 mg/mL) is required and need to dissolve up to 20 uL to prepare the solution of 70 mg/mL.

For dissolution you can use the water (or any other solution) provided.

Please let me know, if you have any doubt by commenting below the answer.

Thanks