Question

Please show work:

1) Complete the calculations and fill in the missing volumes needed below to create a six-tube solute dilution series from a protein stock solution, 1.0 mg/ml BSA (bovine serum albumin) into 1.5 ml microcentrifuge tubes using CIVI = C2V2, note V2 is not 1.5ml. A table is provided below to record your efforts. New solutions Tube Concentration Volumes stock protein Dl water Final volume 1.00 mg/ml 250.0 ul 0.0 ul 250.0 ul 250.0 ul 0.80 mg/ml 0.60 mg/ml 0.40 mg/ml 250.0 ul 250.0 ul 250.0 ul 5 0.20 mg/ml 0.00 mg/ml 0.0 ul 250.0 ul 250.0 ul Which tube above can be utilized as your blank for BSA measurements?

Answer 1

- ② 0.8 mg/ml C, Y = C V2 I XX = 0.8 8250 x=200u 200 ul stock protein + soul Dl water. ② 0.6 mg/ml Cv = C , SA ! XX = 0.6 x 250 x= 150,ul 150 ul stock protein + 100 ul Dl water 6 0.4 mg/ml 0 V, - C - I XX = 0.4 x 250 x= 100 ml 100 ul stock protein + 150ul ol water ② 0.2 mg/ml 1. Ci Vi = CV 1 X 2 = 0.2 x 250 x= 50ml 50 ul stock protein + 200ul ol water

New solutions Tube Concentration Volumes stock protein Dl water Final volume 1.00 mg/ml 250.0 ul 250.0 ul 200 ul 0.0 ul 50 ul 250.0 ul 0.80 mg/ml 0.60 mg/ml 100 ul 150 ul 100 ul 0.40 mg/ml 150 ul 250.0 ul 250.0 ul 250.0 ul 250.0 ul 0.20 mg/ml 0.00 mg/ml 50 ul 0.0 ul 200 ul 250.0 ul

The last solution that has no stock protein (0 mg/ml) can be used as a blank for BSA measurements.