Could you please help me figure this out. This is based on the 'protein concentration quantification'...
Could you please help me figure this out. This is based on the 'protein concentration quantification' lab we conducted in class. We did both the Lowry and Bradford Reagent methods.
There were 3 unknown protein concentrations (unknown A = Lactase; unknown B= Insulin; unknown C= BSA). We used 0.5 ml of each protein sample for our unknowns.
Below are my unknown concentrations for the Lowry method:
Unknown A = 225.3 ug/ml
Unknown B = 238.1 ug/ml
Unknown C= 517.5 ug/ml
Bradford method
Unknown A = 186.4 ug/ml
Unknown B = 110.7 ug/ml
Unknown C = 427 ug/ml
1a) Determine the average concentration for each of the unknown solutions based on the average values from your two techniques you used to determine concentration.
b) Based on the average concentration you determined in part A and considering that the original Lactaid tablet was dissolved in 200 mL of H2O, how many milligrams of protein were present in the tablet?
It's one question with 2 parts... I'd greatly appreciate if you answered both. Thank you so much!!
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Could you please help me figure this out. This is based on the
'protein concentration quantification'...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples:...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
Could you please help me to solve these question with all parts, including graph? thanks so...
Could you please help me to solve these question with all
parts, including graph? thanks so much!!
We were unable to transcribe this imagenumber of drops volume of solution, mL concentration, drops mL-1 (Eq. 6) For example, if the solution you use to determine the spectrum of the food dye, which we shall call "Solution 1," was prepared by adding 1 drop of food dye to 40.0 mL of distilled water, the concentration of the solution is found using Equation...
Hello! Help please, I THINK that's the figure based on what I could find out and...
Hello! Help please, I THINK that's the figure based on what I
could find out and based on the question, but my professor never
gave us Chapter 13 to read for. Regardless, the first question has
got me confused. What experiments exactly? The second question I
can do on my own but I'm unsure if I have the right idea. Thank you!
Essay Questions (30 points): Answer in full sentences, include textbook page numbers to support your information, and reference...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Could you please help me with the questions I got wrong? If you dont know the...
Could you please help me with the questions I got wrong? If you
dont know the answer please do not comment, leave it for another
expert to answer because I really need to get this done , Thank you
so much ^^ There's also a guide to the calculations but i dont get
it
Table view List view Sample 2 Sample 3 Table 5. Calculations for concentration of acid solution Sample 1 [1] Volume of acid (mL) 25.0 25.0 25.0...
Help on prelab NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
In this lab, you will quantitatively estimate the protein concentration in the following 3 unknown samples: 1. Milk 2. Diet drink 3. High protein health food drink. Before you do the lab, you are expected to know the following: 1. How will you make the dilutions of the provided standard protein BSA (Bovine Serum Albumin) in the microfuge (1.7 ml small) tubes? Draw the chart for the same. 3 marks 2. How will you make the dilutions for the above...
Could you please help me to solve these question with all
parts, including graph? thanks so much!!
We were unable to transcribe this imagenumber of drops volume of solution, mL concentration, drops mL-1 (Eq. 6) For example, if the solution you use to determine the spectrum of the food dye, which we shall call "Solution 1," was prepared by adding 1 drop of food dye to 40.0 mL of distilled water, the concentration of the solution is found using Equation...
Hello! Help please, I THINK that's the figure based on what I
could find out and based on the question, but my professor never
gave us Chapter 13 to read for. Regardless, the first question has
got me confused. What experiments exactly? The second question I
can do on my own but I'm unsure if I have the right idea. Thank you!
Essay Questions (30 points): Answer in full sentences, include textbook page numbers to support your information, and reference...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Could you please help me with the questions I got wrong? If you
dont know the answer please do not comment, leave it for another
expert to answer because I really need to get this done , Thank you
so much ^^ There's also a guide to the calculations but i dont get
it
Table view List view Sample 2 Sample 3 Table 5. Calculations for concentration of acid solution Sample 1 [1] Volume of acid (mL) 25.0 25.0 25.0...
Help on prelab
NOTE! There are FOUR (4) pages in this pre-lab! PRE-LAB WORKSHEET FOR ENZYME LAB To be completed prior to the online prelab esercise 1 ) Suppose that the diagram below represents what occurs during a chemical reaction e) Which letter points tq the prodact? print e ore , d 3) (c) Which letter points to the enzyme? (d) Which letter points to the enzyme's active site? (e) Which letter points to the enzyme's substrate? Note: Some letters...
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