Question

1. You are studying a mRNA found in E. coli that appears to encode two separate polypeptides (there are two separate starting points of translation). You would like to make these same proteins by putting this mRNA into a human cell. Assuming you have the ability to make any RNA molecule with a particular sequence, what RNA(s) would you synthesize to be able to express the two polypeptides in a human cell. Describe what you would need to add or remove to the RNA(s) 2. What are polyribosomes and are they found in eukaryotes or prokaryotes? 3. Which site within the ribosome is the tRNA found that is attached to the elongating polypeptide?

Answer 1

Solution of 1:-

In humans mRNA have regions flanking it, called as UTR, or untranslated regions. Towards the 5' side you have the 5'cap which a 7-Methylguanosine and Kozak consensus sequence (ACCAUGG) instead of the Shine Dalgarno (so you must remove it) sequence as Ribosome binding site. Further, at the 3' end there must be a Poly A tail.