Question

2) Suppose you have a solution of a protein, which contains a specific Tyr residue that has an actual (measured) pKa of 8.8.
0 0
Add a comment Improve this question Transcribed image text
Answer #1

Accoring to the Henderson-Hasselbulch equation:

pH =  pKa + Log([Tyr.O-]/[Tyr.OH])

i.e. 8.5 = 8.8 + Log([Tyr.O-]/[Tyr.OH])

i.e. Log([Tyr.O-]/[Tyr.OH]) = 8.5-8.8 = -0.3

i.e. [Tyr.O-]/[Tyr.OH] = 10-0.3 = 0.5

Now, [Tyr.O-]/([Tyr.O-] + [Tyr.OH]) = 0.334

Therefore, the required percentage = 0.334 * 100 = 33.4%

Add a comment
Know the answer?
Add Answer to:
2) Suppose you have a solution of a protein, which contains a specific Tyr residue that...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • show work please 5) Consider the phenolic hydroxyl group of a particular Tyr residue in a...

    show work please 5) Consider the phenolic hydroxyl group of a particular Tyr residue in a protein. Suppose the hydroxyl group in the unfolded protein in aqueous solution, where the group is exposed to H20, has a pka of 10.0. If that group is found in a hydrophobic environment in the interior of the protein when the protein is folded into its native tertiary structure, would you expect the pka of the phenolic hydroxyl to be higher or lower in...

  • Suppose a drug molecule binds to a protein target. Complete the table which describes possible noncovalent...

    Suppose a drug molecule binds to a protein target. Complete the table which describes possible noncovalent interactions (NCT) in the binding site. Assume the drug has a variety of types of polar and nonpolar regions. Type of NCI Species able to form that NCI Parameters affecting the NCI ions; charged species dipole-dipole any hydrogen bond strength and orientation of dipole polarizability, size: molecular weight species with permanent dipoles (C=0, etc.) H bonded to N, O, or F and a dielectric...

  • please answer all. 14. [6 pts) Indicate which of the following statements is True (T) or...

    please answer all. 14. [6 pts) Indicate which of the following statements is True (T) or False (F) When water interacts with hydrophobic molecules, it becomes more ordered and entropy of the system increases The hydrophobic effect is predominant in protein stability The Ramachandran ploy shows combinations of dihedral angles in a polypeptide chain. A proteins function is directly related to the protein's secondary structure. The B sheet is primarily stabilized by R group interactions Clusters of secondary structures are...

  • 1 2 3 4 5 6 7 8 9 Part A Which of the following is/are...

    1 2 3 4 5 6 7 8 9 Part A Which of the following is/are means whereby a catalyst can lower the activation energy of a reaction? quantum tunneling decreasing the number of reactive molecules permanently binding substrates inefficient collisions altering the temperature within the cell to one appropriate for reactions to proceed Subrnit Request Answer Part A An enzyme Obinds substrates in a manner that facilitates the formation of product decreases the rate of a reaction. is always...

  • 8.) which of the following statements does not describe heterotrimeric g proteins involved in the adenylate...

    8.) which of the following statements does not describe heterotrimeric g proteins involved in the adenylate cyclase signaling pathway? a. binding to adenylate cyclase triggers the hydrolysis of PIP2 b. consist of three subunits, Galpha, Gbeta, and Ggamma c. can function as a mediator to adenylate cyclase activity d. are anchored to the cytoplasmic side of the membrane e. activates adenylate cyclase by binding to its C2a subunit 9.) which of the following is not associated with the cAMP binding...

  • IDIOSY CORE Nne Lecture test 2 on the chemicals of Lite Name: 51. Picture a globular...

    IDIOSY CORE Nne Lecture test 2 on the chemicals of Lite Name: 51. Picture a globular protein in the cytoplasm of a cell. Where would you find the following amino acids: alanine and serine? COOCOO A Serine would be in the interior, and alanine would be on the exterior of the globular protein HN-C-H HŇ-Ć-H R Alanine would be in the Interior and serine would be on the exterior of the CHOH CH3 globular protein C Both serine and alanine...

  • please help me type my introduction for my lab report. thank you!! INTRODUCTION In this section,...

    please help me type my introduction for my lab report. thank you!! INTRODUCTION In this section, you will introduce the experiment by explaining generally what you did and why you did it. This section usually starts with an examination of the literature through a library search to inform the reader about work already done on this topic. If you have never done a bibliographic database search for scientific journal articles, then you are not yet a biological sciences major (note:...

  • demic of sleeping sickness. Survivors of 19)A movie called Awakenings dramatized the 1918 epi with a...

    demic of sleeping sickness. Survivors of 19)A movie called Awakenings dramatized the 1918 epi with a drug called dopa. This the disease were left with paralysis and the patients were treated drug has 2 forms: one form relieved example of the paralysis and while the other had no effect. This is an a The fact that each enantiomer can have a different effect in the human body b. When working on developing medications, the researcher needs to be aware of...

  • Titration: Acids and Bases 2. How can you determine which acid is diprotic? 3. using the...

    Titration: Acids and Bases 2. How can you determine which acid is diprotic? 3. using the answers to questions one and two, which acid is diprotic? 4. Which base has more hydroxide ions per molecule? Acid Volume Base Base Initial Volume (mL) Base Final Volume (mL) Volume of Base Used (mL) Acid: Base Ratio Acid 1 20 mL Base 1 50 mL 34.5 15.5 4:3 Acid 2 20 mL Base 1 Acid 1 20 mL Base 2 Acid 2 20...

  • 10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...

    10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT