Let a protein subjected to ultracentrifuge. Given the partial specific volume for a typical globular protein...
Let a protein subjected to ultracentrifuge. Given the partial specific volume for a typical globular protein and the sedimentation coefficient. What fraction of the mass of the particle is experiencing effect after correcting for the buoyancy?
Homework Answers
In an ultracentrifuge rotor rotates at very high speeds, capable of generating acceleration of about 98000000 m/s²
Since this is a very high value the centrifugal force of mv^2/r will be very high and push the particle to the periphery.Buoyancy may cause the particle to rise up but a major fraction of the particle will experience the effect.
Mathematical data would have been helpful in exact calculations
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Let a protein subjected to ultracentrifuge. Given the partial
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Suppose that a force of 1 pN is applied to a globular
100 kDa protein. In the absence of damping, how fast will the
protein be moving after 1 ns? During this time, how far will the
protein have moved? GIven the damping coefficient quoted in Table
2.2, what is the actual terminal velocity of the protein?
Table 2.2 Physical properties of a globular protein of molecular mass To0 Property Mass Density Volume Radius Drag coefficient 60 pN-s/m Diffusion coefficient...
Problems 1-4: For a globular protein of diameter 6 nm, corresponding to a molecular mass of 100 kDa, 1. Calculate the mass in kg. 2. Calculate drag coefficient assuming η_1mPa's 3. Calculate viscous force at 25°C given: The average instantaneous thermal speed of a protein v-(3k*T/m)0.5 k is Boltzmann constant, 1.381 x 10-23 J/HK Tis the absolute temperature, 0 K-273.15°C 1J 1 N'm. 4. Calculate the speed of the protein caused by the centrifugal force. Assume that the centrifugal force...
from the given data sheet, can anyone please let me know how
to solve these post lab questions ?
Post-Lab Questions 1. Calculate the volume of Solution A that you would need to make 50.00 mL of NaOH solution with the same concentration as solution F. Given that one drop of a liquid is 0.05 ml, how many drops of Solution A would be needed? 2. In general, what effect would diluting a NaOH solution have on the pH of...
Describe your solutions in detail, because partial credit will be given based on your work. If additional information is necessary in solving the problems, DO use your engineering sense and provide reasonable assumptions and explanations. You may use your calculator if necessary. Use a language of your choice. Note: The following information may be used. Gas constant, R-8.314 J(mol K)-8.314 kPa L/(mol K)-0.082 L atm/(mol K)-6.132 lbr ft/(gmol K)=1,987 cal/(mol K). Atomic mass of N-14; Atomic mass of O= 1...
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