Question

An article by Sean Decatur published in Accounts of Chemical Research in 2006 details how isotope-edited infrared spectroscopy can be used to elucidate residue-level structure and dynamics of polypeptides. His study focused on using isotopic labeling of a carbonyl functional group. Calculate the wavenumber for (a) a carbonyl stretch with 1C=160, and (b) a carbonyl stretch with 13C-18O.

Answer 1

к фол С-о вon d 3 1. 23 XIо Nn 2пе -12 1 53 XI0 S/om 2x3-14X3X1d 2Пс uM,M2 М,+M2 13х103 Ea lmo9 MB 16 -3 13X10 Imo9 602x13 atoma/mok -26 2.16 X I0 М — 16 XIо 6 02X1023 18 MC Е 1ВX03 6-02 X I 3 ю1g. 2.99X lO Xi0 -26 Mcl2-d8 - 2.16Xiд х 299X06 (2.16+2.99 Xlo зу28 26 26 12XIO Рс3-o16 3 2. 16 x10°х 2.7хI 7 (е16+ 23) х19 26 26 1254XIO

53X1012,1-23xIo3 Nem 12지026 123xlo3 N/em y 1-697 x1o em -1697 em N 인로이8 2 5.3X10 |123시03 N/m 1-254X1o 26 k = -659 X103em = 1659 em FeB-ol6 = 16970m, 인-06 =1659o 1l8 = l6 5 9 e