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Untitled.sp - 9 X Analyze Solution Concentration Time Analyze Light Show Journal Snapshots Absorbance/Transmittance FluoresceUsing given Concentration and Absorption , graph to show the absoprtion spectra of CoCl2. Graph the absorption asa function of wavelength.

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Solution:

Plot the colorimetric assay curve from given values of absorbance and concentration i.e.

Concentration (mg/L) Absorbance
0.200 0.615
0.150 0.486
0.100 0.318
0.050 0.139

Colorimetric Assay (y = 3.192x-0.0095) Concentration (x axis) Absorbance (y axis) 0.200 0.615 0.150 0.486 0.100 0.318 0.050 0

When you plot the curve , you will get a straight line equation

i.e y = 3.192x - 0.0095

now solve for y = 0.643

i.e. 0.643 = (3.192x) - 0.0095

x = (0.643+0.0095) / 3.192

x = 0.204

So 0.204 mg/L is the concentration at absorbance of 0.643

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