Hello all, we did a lab about column chromatography, objective was Separation of a Fluorene and Fluorenol mixture.
Please answer the following (do not write in cursive):
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Hello all, we did a lab about column chromatography, objective
was Separation of a Fluorene and...
Hello all! We did a lab about column chromatography, objective was Separation of a Fluorene and Fluorenol mixture. Initi...
Hello all! We did a lab about column chromatography, objective was Separation of a Fluorene and Fluorenol mixture. Initially we had 0.20 g of the solid mixture (fluorene and fluorenol) I didn't manage to get a final mass reading so im not sure, please go ahead and make a guess for the following, see what makes sense: > Please go ahead and calculuate final mass of fluorene and fluorenone and the melting points of both
Can you help me answer the questions for the Introduction/Background? 1. Introduced the techniques of thin-layer...
Can you help me answer the questions for the
Introduction/Background?
1. Introduced the techniques of thin-layer and flask column
chromatography. Discuss how a solvent is elected as eluent.
2. Discuss all the applications of TLC. What is it used for?
3. Discuss the mobile and stationary phases of TLC and column
chromatography.
4. Explain how column chromatography can be used to separate a
mixture on a larger scale than TLC.
Post-Lab: Objective State the objective of the experiment in your...
Background In this lab, we are going to learn how to use a powerful separation technique...
Background In this lab, we are going to learn how to use a powerful separation technique called chromatography which can separate compounds that are quite similar to one another. Chromatography is a general type of separation in which a mixture of compounds passes through a stationary phase. Different compounds have different relative affinities for the mixture traveling along the stationary phase, or the mobile phase, and the stationary phase, causing these compounds to separate from one another. More specifically, we’re...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase?...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column /...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions NAME DATE LAB SECTION Answer the following questions in the pre-lab section of your notebook. 1. Which compound, acetone or 1-propanol, has the higher boiling point? Why? 2. Do you expect acetone to be soluble in 1-propanol? Why or why not? Model: For this question, you will be graded on completion, rather than correctness of the model. In the post-lab, you will be asked...
During a liquid chromatography lab, caffeine and theophylline were separated into an octadecyl silica column (C18)...
During a liquid chromatography lab, caffeine and theophylline
were separated into an octadecyl silica column (C18) with a mobile
phase consisting of a mixture of water and methanol.
What is the elution scheme?
b) Reason with current theory as background what is expected to happen
the retention factors in case:
- the methanol content in the mobile phase is increased?
- the flow rate is increased?
- the column is replaced with one consisting of octyl silica (C8)?
- switching...
Can you help me answer the questions for the
Introduction/Background?
1. Introduced the techniques of thin-layer and flask column
chromatography. Discuss how a solvent is elected as eluent.
2. Discuss all the applications of TLC. What is it used for?
3. Discuss the mobile and stationary phases of TLC and column
chromatography.
4. Explain how column chromatography can be used to separate a
mixture on a larger scale than TLC.
Post-Lab: Objective State the objective of the experiment in your...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
Briefly explain how chromatography technique works. What type of chromatography is being used in this lab activity? ___________________________________________________ __________________________________ What are the mobile phase and stationary phase substances/Compounds, which are used in this experiment? Briefly explain the purpose of each phase. ____________________________ ______________________________________ If the following mix of molecules were purified using size exclusion chromatography, what would be the order in which the molecules pass through the opening in the bottom of the column? Mixture containing: Hemoglobin, 65,000 Daltons; Ribonuclease...
1. What is the purpose of column chromatography 2. What is the stationary phase? Mobile phase? 3. What is the purpose of the cotton (glass wool) in column chromatography? 4. What is the purpose of the sand? 5. Why is it important to prevent the column from drying? 6. What can happen if you disrupt your even layer of silica gel? 7. Which compounds come off the column first, polar or nonpolar? 8. Keyon synthesized acetylferrocene (orange) in organic chemistry...
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
Case C Thin Layer Chromatography 1. TOXI-LAB is a screening method that uses: chromatography (column / planar) The first step consists of the extraction of drug metabolites from biological fluids when we place the sample in a test tube containing a mixture of solvents and buffering salts that cause the extraction of basic and neutral drugs. The solvent extract of the sample is concentrated by heat and evaporation, then is deposited onto a small disc of chromatographic media (small circle...
LAB 2A SEPARATION OF LIQUIDS BY SIMPLE DISTILLATION ANALYSIS BY GAS CHROMATOGRAPHY LAB 2A Pre-Lab Questions NAME DATE LAB SECTION Answer the following questions in the pre-lab section of your notebook. 1. Which compound, acetone or 1-propanol, has the higher boiling point? Why? 2. Do you expect acetone to be soluble in 1-propanol? Why or why not? Model: For this question, you will be graded on completion, rather than correctness of the model. In the post-lab, you will be asked...
During a liquid chromatography lab, caffeine and theophylline
were separated into an octadecyl silica column (C18) with a mobile
phase consisting of a mixture of water and methanol.
What is the elution scheme?
b) Reason with current theory as background what is expected to happen
the retention factors in case:
- the methanol content in the mobile phase is increased?
- the flow rate is increased?
- the column is replaced with one consisting of octyl silica (C8)?
- switching...
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