During an experimental workup you determine that k2 = 1200 s-1 . When you set [ET]...

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During an experimental workup you determine that k2 = 1200 s-1 . When you set [ET]...

During an experimental workup you determine that k2 = 1200 s^-1 . When you set [E_T] = 10 nM and [S] = 40 μM, you observe Vo progressing at 9.6 μM s^-1 . For this reaction, what is K_M?

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Answer 1

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Solution Given ) K = 12006-1 [ET] = 10nM [s] - 40nM Vo = 9.6uM5 To find kmi Firest of all we need to find max. max = K₂ [ET] the enzymatic reaction related formulas have been used to solve the above question.

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During an experimental workup you determine that k2 = 1200 s-1 . When you set [ET]...

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