Question

1. Show, using the Michaelis-Menten equation, that when [S] >>> Km, vo = Vmax. Show, using the M-M equation that when [S] <<<K_m, v_o =[S][E_t]k_cat/K_m.

2. What is V_max? Provide both a mathematical and written description of V_max? How can V_max be experimentally altered? How can we use V_max to determine the turnover number (k_cat) of an enzyme-catalyzed reaction? What is the major challenge of determining V_max from an Michaelis-Menten plot?

Answer 1

1.) Michaeling-Menten equations u= max [] K+ [s] Cax I: when [s] »km , then we can write [s] + km 2 (s] So, vo= man es may max ma Case I: [s] <<< km, then we can write [s] + km ~ km vo= max [s ] Vmax = Kear [Enzyme] lvo - Kear [Engen] [s)