What is the velocity of a Michaelis-Menten enzyme reaction (in terms of vmax) when the concentration...
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What is the velocity of a Michaelis-Menten enzyme reaction (in
terms of vmax) when the concentration...
b. For an enzyme that displays Michaelis-Menten kinetics, what is the initial velocity as a function of Vmax when: a. [...
b. For an enzyme that displays Michaelis-Menten kinetics, what is the initial velocity as a function of Vmax when: a. [S] Km b. S] 0.1 Km c. [S] 50Km c. What will be the initial velocity (yo) for an enzyme that has Km 2.5 [S]? Your answer will be a fraction of Vmax a.
The relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation a) At what substrate concentration would...
The relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation a) At what substrate concentration would an enzyme with a kcat of 30.0 s-1 and a Km of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: [S]=Km/2, [S]=2Km, [S]=10Km
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point...
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point where the enzyme is working at its maximum rate (Vmax/2), and amount of substrate needed to bind half of the active sites (Km). Label these points on the graph. Vmax represents: Vm Vmax/2 represents: Reaction velocity v Vmax 2 Km represents: Kim Substrate concentration (5)
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate ...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
For an enzyme that displays Michaelis-Menten kinetics, what is the reaction velocity v (as a percentage...
For an enzyme that displays Michaelis-Menten kinetics, what is the reaction velocity v (as a percentage of Vmax) ,observed at each of the following substrate concentrations. (Ex, v = xVmax, where x = an integer, fraction, or decimal number (two decimal places)) a) [S] = 0.1 Km _________ b) [S] = 2 Km _________ c) [S] = 10 Km _________
9. Applying the Michaelis-Menten Equation I An enzyme catalyzes the reaction A = B. The enzyme is present at a con- cen...
9. Applying the Michaelis-Menten Equation I An enzyme catalyzes the reaction A = B. The enzyme is present at a con- centration of 2 nm, and the Vmax is 1.2 ums". The Km for substrate A is 10 um. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 um, (b) 10 um, (C) 30 um.
will rate thanks Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS...
will rate thanks
Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS -MENTEN GRAPH, WHY DOES THE CURVE REACHES PLATEAU? Vmax Reaction velocity (v) Vm/2 Km Substrate concentration (S) Q3. IN MICHAELIS MENTEN GRAPH, HOW WOULD YOU INCREASE VELOCITY BEYOND Vmax? Q4. SMALLER VALUE OF THE MICHAELIS CONSTANT (Km) REFLECTS HIGHER EFFICIENCY OF THE ENZYME. (TRUE/FALSE).
For an enzyme that follows the Michaelis-Menten kinetic, what substrate concentrations (relative to Km) are needed...
For an enzyme that follows the Michaelis-Menten kinetic, what substrate concentrations (relative to Km) are needed for the speed of the reaction to be 0.12 there vmax 0.25 there vmax 0.5 there vmax 0.9 there vmax.
1. Show, using the Michaelis-Menten equation, that when [S] >>> Km, vo = Vmax. Show, using...
1. Show, using the Michaelis-Menten equation, that when [S] >>> Km, vo = Vmax. Show, using the M-M equation that when [S] <<<Km, vo =[S][Et]kcat/Km. 2. What is Vmax? Provide both a mathematical and written description of Vmax? How can Vmax be experimentally altered? How can we use Vmax to determine the turnover number (kcat) of an enzyme-catalyzed reaction? What is the major challenge of determining Vmax from an Michaelis-Menten plot?
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed react...
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. S Where v is the velocity or rate, Vmax is the maximum velocity, Km is the +IST Michaelis- Menten constant, and I5 s the substrate concentration. K + S v (uM/min) a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (Vo) at different substrate concentrations ([S]) 300 Vmax 250 1/2 Vmax First, move the line labeled "Vmax to a...
b. For an enzyme that displays Michaelis-Menten kinetics, what is the initial velocity as a function of Vmax when: a. [S] Km b. S] 0.1 Km c. [S] 50Km c. What will be the initial velocity (yo) for an enzyme that has Km 2.5 [S]? Your answer will be a fraction of Vmax a.
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point where the enzyme is working at its maximum rate (Vmax/2), and amount of substrate needed to bind half of the active sites (Km). Label these points on the graph. Vmax represents: Vm Vmax/2 represents: Reaction velocity v Vmax 2 Km represents: Kim Substrate concentration (5)
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
9. Applying the Michaelis-Menten Equation I An enzyme catalyzes the reaction A = B. The enzyme is present at a con- centration of 2 nm, and the Vmax is 1.2 ums". The Km for substrate A is 10 um. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 um, (b) 10 um, (C) 30 um.
will rate thanks
Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS -MENTEN GRAPH, WHY DOES THE CURVE REACHES PLATEAU? Vmax Reaction velocity (v) Vm/2 Km Substrate concentration (S) Q3. IN MICHAELIS MENTEN GRAPH, HOW WOULD YOU INCREASE VELOCITY BEYOND Vmax? Q4. SMALLER VALUE OF THE MICHAELIS CONSTANT (Km) REFLECTS HIGHER EFFICIENCY OF THE ENZYME. (TRUE/FALSE).
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. S Where v is the velocity or rate, Vmax is the maximum velocity, Km is the +IST Michaelis- Menten constant, and I5 s the substrate concentration. K + S v (uM/min) a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (Vo) at different substrate concentrations ([S]) 300 Vmax 250 1/2 Vmax First, move the line labeled "Vmax to a...
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> Where did you get the 5 from?
Humberto Calderon Tue, Nov 9, 2021 8:02 PM