Question

9. Applying the Michaelis-Menten Equation I An enzyme catalyzes the reaction A = B. The enzyme is present at a con- centration of 2 nm, and the Vmax is 1.2 ums". The Km for substrate A is 10 um. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 um, (b) 10 um, (C) 30 um.

Answer 1

4Moito 1b G 0rly Reaction geven hoo3 odals A B concentration et enzyme Te] = 2 nM -I Vmax 1.2 - UM S 5O HO for A 10 RLM Km FOTA Michaelis- Menten eguattonis JoiJows: give as Vman sJ Siat [SJ+ Km No=initial rate Subst qaie cm, T Km Rate const. tor the meaction Vmasmaximal rate

dE. [5] 2.0M whern a iM 1.2 MMS X 2eM + 1 uM []+ Kn 12 uMSX 2 uM 12 uM O.1X2 RIMS 0, 2 MS dhen [S] 10 uM Vmax 1.2 iMS 10.UM [] Km 108M t10M 12 LAMS 20 VD 0.6 L0MS when [ 30 IM Vmax .22iMS x 30M [5] tKm 3DMM 10LM I2 x 3 M 4 O9

should be noted that, in orger to pply Michaelis- Menten rinetics thu Concenfaation nst be much lorer than the Substrate concenfoation eneyn