Question

2nd attempt MS Characteristics (9 items) (Drag and drop into the appropriate area below No more items Mass Spec Types 19 OF 21 QUESTIONS COMPLETED <10/21 VIEW SOLUTION SUBMIT ANSWER & 7 2 4 6 5 B W R E T Y P U D F G J K L Z V В option command command option A

Answer 1

ESI :

Sample becomes ionized due to high voltage

Degrades and charges peptides with high voltage

Ionization evaporates solvent leaving peptides in gas phase to travel through the separating mass spectrometer

MALDI:

Peptides become fragmented and charged due to laser exposure

Laser will be absorbed by the peptide containing material releasing peptide into gas phase

Peptides and proteins are mixed in solid matrix rather than solution

Both ESI and MALDI:

Uses mass to charge ratio to determine molecular mass

Based on mass and acceleration through chamber ending in detector

Can be used for proteins and peptides treated with proteases like trypsin