Question

Cheach characteristic to the appropriate chromatography technique. Description (11 items) (Drag and drop into the appropriate area below) Antibodies are sometimes used to bind target proteins in this chromatography Small proteins are retained due to a longer pathway through the column. Metal ions can be fixed to a resin and bind to specific sequences of amino acids such as histidine Uses a porous bead made of complex carbohydrates that allow proteins to go through or around Two proteins of the same size but different shape Iglobular vs fibrous) can be separated by this method Separates proteins based on specific binding properties of the target protein Exploits the charge state protein to ad to a chromatogray resin Chromatography Technique Cel Filtration Poror VIEW SOLUTION SUBMIT ANSWER < 03/21 > OF 21 QUESTIONS COMPLETED P w LEIRITTO Option COMO opron command

Answer 1

Antibodies are generally used to bind target protein in this chromatography- Affinity chromatography

small proteins are retained due to longer pathway through the column - Gel filtration chromatography

metal ions can be fixed to a resin and bind to specific sequences of amino acid such as histidine - Ion exchange chromatography

uses a porous bead made of complex carbohydrate that allow protein to through or around - Gel filtration chromatography

Two proteins of same size but different shape(globular vs fibrous)can be separated by this method - High performance Gel filtration chromatography

separates protein based on the specific binding properties of the target protein - Affinity chromatography

exploits the charge state of a protein to adhere to a chromatography resin - Ion exchange chromatography

natural or mimicked ligand of the target protein is bound to a solid phase resin,often a dextran bead - Affinity chromatography

proteins are displaced from the resin by changing pH or increasing ionic strength(salt conc) of the elution buffer- Ion exchange chromatography

DEAE a positive charged resin used in this type of chromatography - Ion exchange chromatography

can be used to determine molecular mass of an unknown protein using a set of proteins with known molecular weight - Gel filtration chromatography