Question

13.2 mMCm- 3. If the initial, linear portion of the line has a slope (Aabsorbance/Atime x cm) of 0.02/second, use the extinction coefficient above to convert this to a slope that gives the AIDHF|/Atime. Remember to include units in your answer. Note: you can use Beer's law in the following way: A(absorbance) E 0.5 cm * A[DHF]| Note: Solve for AIDHF]!

Answer 1

Question 3.

According to Beer's law:

$\Delta$(absorbance) = $\epsilon$ * 0.5 cm * $\Delta$[DHF]

i.e. $\Delta$(absorbance)/$\Delta$time = $\epsilon$ * 0.5 cm * $\Delta$[DHF]/$\Delta$time

i.e. 0.02/sec = 13.2 mM^-1.cm^-1 * 0.5 cm * $\Delta$[DHF]/$\Delta$time

i.e. $\Delta$[DHF]/$\Delta$time = 0.00303 mM/sec or 3.03 $\mu$M/sec