You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice?
A. Shelf life of reagents
B. Speed of detection
C. Wavelengths detected by microtiter plate reader
D. Sensitivity
Which assay format is the best choice for measuring small molecules, such as drugs and small peptide hormones?
A. Latex agglutination
B. Fluorescence polarization
C. Capture ELISA
D. immunochromatographis assay
Point-of-care testing is performed most easily using:
A. homogeneous enzyme immunoassay.
B. immunofixation assay.
C. immunochromatographic assay.
D. capture ELISA.
A tissue section is fixed to a microscope slide, then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue. The preparation is then washed and inspected with a fluorescent microscope. This technique is called:
A. direct immunofluorescence.
B. indirect immunofluorescence.
C. fluorescence polarization.
D. autofluorescence.
The signal measured in a fluorescence polarization immunoassay is the:
A. wavelength of absorbed light.
B. angle of light scattering.
C. intensity of light emission.
D. orientation of emitted light waves.
Which compound would be an appropriate label in a chemiluminescent assay?
A. Horseradish peroxidase
B. Iodine-125
C. Fluorescein
D. Ruthenium derivative
Quenching of the signal emitted by the label is of particular concern in:
A. radioimmunoassay.
B. enzyme immunoassay.
C. fluorescence polarization.
D. chemiluminescent immunoassays.
Pregnancy testing can be performed at home using:
A. capture assay.
B. immunochromatography.
C. fluorescence polarization.
D. chemiluminescence.
An antigen is fixed to a microscope slide, then incubated with patient serum. After washing, fluorescently labeled anti-human immunoglobulin is applied. After an additional wash, the slide is inspected with a fluorescent microscope. This technique is called:
A. direct immunofluorescence.
B. indirect immunofluorescence.
C. fluorescence polarization.
D. autofluorescence.
colorimetric enzyme immunoassay kit to measure a plasma protein that circulate at moderately high concentration. each kit uses a different enzyme and substrate
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a...
23. Point-of-care testing is performed most easily using A. homogeneous enzyme immunoassay. B. immunofixation assay. C. immunochromatographic assay. D. capture ELISA.
help thank you! 28. All of the following would be considered good quality control protocols except: a. storing reagents properly b. allowing the reaction to go as long as possible. c. checking temperatures of water baths, etc. d. running positive and negative controls. 29. What would best characterize an indirect immunofluorescent assay when testing for the presence of a specific antibody in the patient's specimen. a. The anti-human immunoglobulin has a fluorescent label. b. The patient's serum is tagged with...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what was done A summary of the most important results A sentence stating the major conclusion EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...