Determine the pH of an aqueous buffer solution (250 mL) comprised of dissolving 5.2812 g of Tris (B) (FW = 121.14 g/mol) and 10.2500 g of Tris-HCl (BH+) (FW = 157.60 g/mol) if pKa (BH+) = 8.07?
(a) 8.24 (b) 7.90 (c) 8.54 (d) 7.82 (e) 8.01
The pH can be calculated using the Henderson-Hasselbach equation:
We need to calculate the concentrations of the species, which can be done using:
For B:
For BH+:
And finally, we can calculate:
Determine the pH of an aqueous buffer solution (250 mL) comprised of dissolving 5.2812 g of...
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