4. A buffer you want to use for protein purification calls for the following components: Tris-HCI...

Question

Question

4. A buffer you want to use for protein purification calls for the following components: Tris-HCI (110 mM, MW = 121.14 g/mol)

Answer 1

Answer #1

IN the lab we have 500mM TrisHCL so to make it 110mM for 2500 ml we have to use 110*2500/500=550ml

similarly we have 125 mM B-mercaptoethanol, to make 50 mM we have to use 50*2500/125=1000ml

so to prepare the reagent we have to use-

11.25 gram urea

111.57 gram NACl

1.54 gram Dithiotheitol

550ml of Tris HCl from the stock

1000 ml of B-mercaptoethanol from the stock

and them makes of the final volume 2500ml with distilled water

Answer 2

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