Question

Calculate the isotonic and isoelectric pH of 0.03479 M tryptophan. Enter your answers to the hundredths place. phai p2 Amino Acid Asparagine Glutamine 2.16 2.19 9.00 isolonic pH = Isoleucine 2.318 9.758 2328 9.744 9.08 isoelectric pH = Leucine Methionine Phenylalanine Proline Serine Tryptophan Valine 2.18 2.20 1.952 9.31 10.640 2.187 9.209 2.37 9.33 2.286 9.719

Answer 1

Isoionic point [H+] = √ [(K1K2F + K1Kw) / K1 + F)]

[H⁺] = √ [(10^-2.37 x 10^-9.33 x 0.03479) + 10^-2.37 x 1.0 x 10^-14) / (10^-2.37 + 0.03479)] = 0.00000133357

pH = -log[H⁺] = -log[ 0.00000133357 ] = 5.87

pH = 5.87 for isoionic point.

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Isoelectric point pI = 1/2 (pK_a1 + pKa₂) = 1/2 (2.37 + 9.33) = 5.85

Isoelectric pH = 5.85

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Calculate the isoionic and isoelectric pH of 0.03479 M Amino Acid phai рк tryptophan. Enter your answers to the hundredths place. Asparagine 2.16 8.73 Glutamine 2.19 9.00 isolonic plH5.87 Isoleucine 2.318 9.758 Leucine 2.328 9.744 Methionine 2.18 9.08 isoelectric pH=5.85 Phenylalanine 2.20 9.31 Proline 1.952 10.640 Serine 2.187 9.209 Tryptophan 2.37 9.33 Valine 2.286 9.719

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