Which of these is a constitutive mutant?
a. lacI-
b. lacOc
c. lacIs
d. All of the above
e. Both A & B
Ans -e
lacl-
lacOc
these two are constructive mutant of lac operone
they fail to stop lac operone In constitutive media lacking lactose
Which of these is a constitutive mutant? a. lacI- b. lacOc c. lacIs d. All of...
Illustrate a conjugation event with an F’ cell that is lacIS lacP+lacO+lacZ- lacY-on the bacterial chromosome and lacI- lacP+ lacOC lacZ+ lacY+ on an F plasmid with an F-cell that is lacI+ lacP+ lacO+ lacZ+ lacY-. Draw both cells before the conjugation event, as well as the recipient cell after conjugation occurs. Underneath all three cells, note whether the production of permease and beta-galactosidase would be high, basal, or none, and describe HOW the proteins and DNA sequences interact to...
A bacterium has the following genotype: lacIS lacP+ lacO- lacZ+ lacY- / lacI- lacP- lacO+ lacZ- lacY+ Fill in the blanks with either “on” or “off”. a. In the presence of lactose, transcription of β-galactosidase is __ON____. b. In the absence of lactose, transcription of β-galactosidase is ___ON___. c. In the presence of lactose, transcription of permease is __OFF____. d. In the absence of lactose, transcription of permease is __OFF____. Please give a detailed explanation....the mutations such as lacI^s are...
Which of these is considered allosteric? a. LacO b. LacI c. LacZ d. LacY e. LacA
You isolate 20 different OC mutant strains that are wild type for all other components of the Lac Operon. You observe that when grown on lactose all of the strains exhibit a relative B-gal concentration of 100. When each strain is grown on glucose they all exhibit constitutive expression of B-gal, but the different strains exhibit different Bgal concentrations that range from 20 to 100. Which of the following could account for these observations? a) the OC mutant strains each...
A Bar body is an example of what? A) Constitutive euchromatin B) Facultative heterochromatin c) Constitutive heterochromatin D) Facultative euchromatin What were the conclusions of Franklin's work regarding the structure of DNA? A) The helix contained about 1 bases pertum B) The helix had more than one strand. c) The helix had only one strand D) It did not have a helical structure. 3) According to Chargaff's nie, if the DNA of a species contains 20% adenine, what percent of...
The E. coli Fur protein is - a constitutive protein. - a regulatory protein. - an allosteric protein. - a repressor protein. A. One of the above. B. Two of the above. C. Three of the above. D. All of the above.
which operon will make b-galactosidase even if lactose isn't present? p+ o+ lacZ+ lacI- p- Oc lacZ+ lacI- p+ Oc lacZ- lacI+ p- o+ lacZ+ lacI+ p+ o+ lacZ+ lacI+ Oc = fails to bind R regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
Whicts b. B ?.? d. D e. All of these exeept C 14. Which of the following does not represent a similarity between nitriles and carboxylic ac a. contain 3 carbon bonds to an electronegative element b. e. d. e. all of these are characteristic of both nitriles and carboxylic acids. contain two st bonds. act as electrophiles undergo nucleophilic substitution reactions 15. Which of the following is the correct assignment of the classes of the following compounds? CH3 a....
The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription of all the genes within the lac operon. In this case, LacI is called a transcriptional _______. In comparison, CRP binds to the CRP binding site sequence upon binding of cAMP, enhancing transcription. CRP is called a transcriptional _______. Select one: a. activator ;;;;; activator b. activator ;;;;; repressor c. repressor ;;;;; activator d. repressor ;;;;; repressor e. None of these