The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription...
The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription of all the genes within the lac operon. In this case, LacI is called a transcriptional _______. In comparison, CRP binds to the CRP binding site sequence upon binding of cAMP, enhancing transcription. CRP is called a transcriptional _______.
Select one:
a. activator ;;;;; activator
b. activator ;;;;; repressor
c. repressor ;;;;; activator
d. repressor ;;;;; repressor
e. None of these
Homework Answers
C is absolutely correct answer because lacI product is repressing the expression and CRP activates expression of lac operon Gene.
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The LacI protein is released from the operator sequence upon
binding of IPTG, allowing the transcription...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
In the lac operon, what are the likely effects on operon transcription of the mutations listed...
In the lac operon, what are the likely effects on operon transcription of the mutations listed below? PLEASE EXPLAIN your answers. I think I answered the first part of the question but need help explaining why. Mutation of the consensus sequence in the lac promoter. i. Transcription is blocked. Mutation of the repressor binding sites in the operator sequences. i. Transcription is constitutive. Mutation of the lacI gene affecting the allosteric site of the protein. i. Transcription is blocked. Mutation...
Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it...
Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it from binding to the Operator on the lac operon. But if the repressor is on the operator, no B-glactosidase can be made which means no allolactose can be made in the first place. So how is the repressor removed so allolactose can be made to stop the repressor from binding?
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Molecular Biology Help! In positive regulation, a(n) ______________ activates a promoter. activator repressor enhancer regulon none...
Molecular Biology Help! In positive regulation, a(n) ______________ activates a promoter. activator repressor enhancer regulon none of the above In negative regulation, a(n) _____________ turns off the transcription of one or more genes. activator repressor enhancer regulon none of the above The _______ activator protein regulates the araBAD operon. allolactose cAMP-CRP AraC enhancer none of the above Three _____ protein binding sites increase rrn transcription. promoter Fis AraC cAMP-CRP none of the above
Match each term associated with genes and control of gene expression with the appropriate description. A...
Match each term associated with genes and control of gene expression with the appropriate description. A transcriptional unit" that consists of promoter multiple genes under the control of a single regulatory element. A transcriptional regulatory protein (prokaryotic or eukaryotic) which works by turning on or increasing gene transcription. activator The region of a gene to which RNA polymerase binds. Enhancer A transcriptional regulatory protein prokaryotic or eukaryotic) which works by turning off or decreasing gene transcription. repressor A molecule that...
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding...
A) An Operon is:? 1- A gene that affect another gene transcription. 2. A gene coding for a metabolic pathway. 3. A protein that blockgene expression. 4. A set of genes under the control of one promoter. B )- In bacteria the Lac Operon genes code for proteins that break down.? 1- Arabinose 2- Lactose 3- Galactose 4- Glucose C )mRNA processing involves removal of .......? 1- Introns 2- The poly-A tail 3- The 5' cap 4- Exons D )...
Match the protein listed below with the gene that encodes it or with the DNA sequence...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
The type of regulator that binds an operator is called a(n) and usually binds to a sequence...
The type of regulator that binds an operator is called a(n) and usually binds to a sequence on the DNA located the RNA polymerase binding site. activator / overlapping activator / adjacent to repressor / adjacent to repressor / overlapping
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon?...
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon? CAMP is a repressor of the lactose operon and turns off gene expression of the lactose operon CAMP is an activator and prevents the repressor protein from binding to the operator region of the operon which allows for the lactose operon to be turned "on". 5 CAMP is an inducer of the lactose operon and also an activator since it prevents the repressor protein...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
Match each term associated with genes and control of gene expression with the appropriate description. A transcriptional unit" that consists of promoter multiple genes under the control of a single regulatory element. A transcriptional regulatory protein (prokaryotic or eukaryotic) which works by turning on or increasing gene transcription. activator The region of a gene to which RNA polymerase binds. Enhancer A transcriptional regulatory protein prokaryotic or eukaryotic) which works by turning off or decreasing gene transcription. repressor A molecule that...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
Question 12 2 pts What is the role of CAMP in regulation of the lactose operon? CAMP is a repressor of the lactose operon and turns off gene expression of the lactose operon CAMP is an activator and prevents the repressor protein from binding to the operator region of the operon which allows for the lactose operon to be turned "on". 5 CAMP is an inducer of the lactose operon and also an activator since it prevents the repressor protein...
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