Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it...
Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it from binding to the Operator on the lac operon. But if the repressor is on the operator, no B-glactosidase can be made which means no allolactose can be made in the first place. So how is the repressor removed so allolactose can be made to stop the repressor from binding?
Homework Answers
Cells always have a basal level of β-galactosidase.
Upon increase/introduction in lactose levels inside the cells, this basal amount of β-galactosidase converts few molecules of lactose into allolactose which binds to Lac repressor. Binding of allolactose to Lac repressor prevents the repressor from binding to the lac operon operator thereby promoting its expression.
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Allolactose acts as a signal molecule that binds to the LacI
protein (repressor) and prevents it...
3. Mutations in bacteria that affect gene regulation may have a cis- or trans-effect on the...
3. Mutations in bacteria that affect gene regulation may have a cis- or trans-effect on the expression of the protein-encoding genes in an operon? How would the following mutations affect the expression of the lac operon? Assume there could be more than one lac operon in the cell and that no glucose is present. A. A mutation in the operator site that prevents the lac repressor from binding. B. A mutation in the lacI gene that prevents allolactose from binding,...
The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription...
The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription of all the genes within the lac operon. In this case, LacI is called a transcriptional _______. In comparison, CRP binds to the CRP binding site sequence upon binding of cAMP, enhancing transcription. CRP is called a transcriptional _______. Select one: a. activator ;;;;; activator b. activator ;;;;; repressor c. repressor ;;;;; activator d. repressor ;;;;; repressor e. None of these
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively.
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene.The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. Determine which events...
1. (1 pt) If a gene is repressible and under positive control, A. Is the regulatory...
1. (1 pt) If a gene is repressible and under positive control, A. Is the regulatory protein an activator or repressor? B. Explain how an effector molecule, which binds to the regulatory protein, alters the regulatory protein’s ability to regulate expression of the gene. 2. (1 pt) Cis and trans are two terms used to describe mutations. A. Explain the molecular difference between these terms. B. indicate which type of mutation (cis or trans) is dominant to wild-type and which...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions,...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
all them please Question 23 (1 point) The A and B alleles in ABO blood types...
all them please
Question 23 (1 point) The A and B alleles in ABO blood types can give rise to an individual that is blog type AB. This specific blood type is an example of: A) multiple alleles B) epistasis C) codominance D) partial dominance Imagine the gene encoding the lac repressor was mutated so that lactose (more technically allolactose) no longer bound to the repressor. However, the lac repressor was still capable of binding DNA at the operator sequence....
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
here is the diagram ecause there is no repressor protein attached to the operator, what enzyme...
here is the diagram
ecause there is no repressor protein attached to the operator, what enzyme con attach to the promoter and move past the operator to transcribe the structural genes? Color this enzyme pink "color the repressor gene purple and the repressor protein it codes for red. Examine the shape of the repressor protein. 45) Is it an active or inactiverepressor protein? The diagram below shows the trp operon when turned off." Repressor gene Promoter Operator Structural genes DNA...
1. Which of the following organisms would produce functional lac products in the presence of IPTG?...
1. Which of the following organisms would produce functional lac products in the presence of IPTG? A. Is Oc Z- Y+ A+ B. Is Oc Z- Y- A- C. I+ O+ Z+ Y+ A+ D. I-Oc Z+ Y+ A+ E. Both Is Oc Z- Y+ A+ and Is Oc Z- Y+ A+ are correct 2. Which of the following statements is true about a lac operon with this genotype? I-d O+ Z+ Y- A- A. The operon is repressible. B....
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
all them please
Question 23 (1 point) The A and B alleles in ABO blood types can give rise to an individual that is blog type AB. This specific blood type is an example of: A) multiple alleles B) epistasis C) codominance D) partial dominance Imagine the gene encoding the lac repressor was mutated so that lactose (more technically allolactose) no longer bound to the repressor. However, the lac repressor was still capable of binding DNA at the operator sequence....
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
here is the diagram
ecause there is no repressor protein attached to the operator, what enzyme con attach to the promoter and move past the operator to transcribe the structural genes? Color this enzyme pink "color the repressor gene purple and the repressor protein it codes for red. Examine the shape of the repressor protein. 45) Is it an active or inactiverepressor protein? The diagram below shows the trp operon when turned off." Repressor gene Promoter Operator Structural genes DNA...
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