Question

If cultured cells were overgrown (1:2 seeding ratio), how to perform subculture experiment differently? also, indicate Procedure in cell subculture.

Answer 1

If we want to subculture a culture which have overgrown, then we have to follow the steps written below -

1. Flame sterilize all the glasswares to be used, needles, inoculating loop and your hands. Wear gloves.

2. Pick up only one Colony using a needle from the overgrown culture.

3. Inoculate this Colony using streak plate method on a fresh media plate.

4. Incubate the plate for 2 to 3 days at appropriate temperature.

5. You can also inoculate the subculture using spread plate method. In this method, you just have to pick up one colony from the overgrown culture and then using the inoculating loop, you have to spread the culture uniformly on a fresh media plate. Then incubate this plate for 2 to 3 days at appropriate temperature.

Subculturing can be performed using streak plate method, pour plate method and event spread plate method. You just need a freshly prepared nutrient media plate and need to incubate the plate for 2-3 days.

streak plate method 2 OOO Oo spread plate method

Please rate high.