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1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible...

1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible reasons and how to improve the specificity.

2.If there was not any band, discuss possible reasons and how to troubleshoot?

3.  If cultured cells were overgrown (1:2 seeding ratio), how to perform subculture experiment differently?

4. Determine the confluence of each of seeding ratio by estimation (1:10 and 1:20 ratio)

5. Procedure in cell subculture?

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