In order to prepare pH = 7.00 of 1.00 L buffer solution, you added 12.43 g tris (MW: 121.135), calculate the mass in g of tris hydrochloride (MW: 157.596) that you will need to add. pKa = 8.072. Show your calculation process.
mass of tris = 12.43 g
moles of tris = 12.43 / 121.135 = 0.1026 mol
pH = 7.00
pKa = 8.072
pH = pKa + log [B / BH+]
7.00 = 8.072 + log [0.1026 / BH+]
moles of BH+ = 1.211 mol
mass of BH+ = 190.8 g
mass in g of tris hydrochloride = 190.8 g
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