are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a)...

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are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the bu

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Answer 1

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m= 200 xlo mol 200x1o 3 mol x 58.58 x 18 mor = [11.7gl Mac 2 m= 100 Xorno1 x 1210 18 XX I m = 12.11g] tris base pky Tris = 5. 2

1. D NG ANTA Buffer V=1L , loomM Tris 200mm Nach, PH=78 Molarity = man mobarmans x volume - a)

3. 1V=65.58mLL They we need to add 65.58 mL of IM HCl to reach the pH = 7.8

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