1) Solution Components
A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4
B 5 mL 100 mM NaH2PO4 + 5 mL 100 mM Na2HPO4
C 9 mL 100 mM NaH2PO4 + 1 mL 100 mM Na2HPO4
D 10 mL 100 mM NaH2PO4 stock solution
E 10 mL 100 mM Na2HPO4 stock solution
F 10 mL distilled water
pKa of phosphate: 6.8
How would you calculate the pH of each equation using the Henderson Hasselbalch equations?
2) Tris buffer (pKa 8.3) is a weak organic base. It’s available
in solid form as “Tris base” (MW =
121.1 g/mol) and “Tris acid” (MW = 157.6 g/mol).
Calculate the theoretical pH of a solution that contains 80 mL of 50 mM Tris base and 20 mL of the 50 mM Tris acid. (my answer = 10.4) At what pH will the acid and base forms of Tris be present at the same concentrations?
My answer was 8.3 but I'm not sure if that's right.
2) # Theoretical pH value is 8.78 for the solution containing 80 mL of 50 mM Tris base and 20 mL of the 50 mM Tris acis.
## At pH 8.3 the acid and base forms of Tris will be present at the same concentrations.
1) Solution Components A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4 B...
a) What are the concentrations of Na2HPO4 and NaH2PO4 in a 0.30 M phosphate buffer solution pH 7.0? Use pKa 6.82 b) Describe how you would prepare 250 mL of the buffer in part (a) given that you have available to you a 1.0 M stock solution of NaH2PO4 and solid Na2HPO4(FW 141.96 g/mol). Provide your answer in milliliters of NaH2PO4 and grams of Na2HPO4. c) Suppose you use 100 ml of this buffer in an experiment and 0.003 mol...
1.Show calculations for making following reagents A. 200 ml of a 1M solution of Tris (molecular weight 121.1 g/mol) B. 300 ml of 0.2M EDTA (MW 372.2 g/mol) C. 200 ML of TE buffer (contains 10 mM Tris and 1 mm EDTA) using solutions made in A and B A solution containing a concentration of 0.01M (H+) ions will have a pH of --- 3. What is the Henderson Hasselbach equation? Use this equation to...
3) Calculate the pH of a solution by mixing 50.0 mL of 0.200 M Na2HPO4 with 20.0 mL 0.0400 M NaH2PO4. (Hint: Buffer solution. Use Henderson-Hasselbalch equation) H3PO4 5 H+ + H2PO4 pkı = 2.1 H2PO4 5 H+ + HPO42- pK2 = 7.2 HPO42- 5 H+ PO43- pK3 = 12.3
Use the Henderson Hasselbalch equation to calculate the volume in mls of a) Tris acid and b) Tris base to use to make 250 ml of a 0.01M Tris buffer, pH 8.0, from 0.01M stock solutions. Give your answer to one place of decimals. pKa Tris = 8.3.
Indicate the appropriate protocol below to prepare the required solution A) Solution 1 Preparation: Prepare: 1 liter of 2 Molar Tris From: solid Tris, MW = 121.1 g/mol Procedure: B) Solution 2 Preparation: Prepare: 1 liter of 0.01 mg/mL ascorbic acid From: solid ascorbic acid , MW = 176.1 g/mol Procedure: C) Solution 3 Preparation: Prepare: 1 liter of 100 mM Tris From: 1 Molar Tris stock solution Procedure: D) Solution 4 Preparation: Prepare: 1 liter of 50 mM CaCl2...
If you have a 20mM solution of NaH2PO4 and you want to make a solution of pH=6.42, what should be the concentration of Na2HPO4 ? The pka of these two forms is 6.82. - Bonus Q: How many grams of Al(SO4)3 18H20 (MW 666.42 g/mol) would you add to 200 ml water to make a 20mm stock solution? sm wolnaza arlok
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
Calculate the pH of a solution made by mixing 100 mL of 0.1 M NaH2PO4 and 300 mL of 0.1 M Na2HPO4.
Each group of students should prepare 100 mL of ONE of the following PAIRS of buffers 2. Phosphate buffer a. 100 mM potassium-phosphate, pH 5.0 b. 100 mM potassium-phosphate, pH 7.0 To prepare 100 mL of 100 mM phosphate buffers by titrating the monobasic form with base, first calculate how much KH2PO4 you need. Dissolve this amount in 50 mL of water. Using the Henderson-Hasselbalch equation, calculate how much 1 M NaOH should be required to achieve the desired pH...
Sample Buffer Calculation: Given a pH of 5.4, prepare a buffer using one of the buffer systems below: System A: 3.5M acetic Acid (MM=60.1) : sodium acetate, anhydrous (MM=82.0) pKa = 4.74 System B: Sodium phosphate, monobasic (NaH2PO4 x H2O) (MM=138.0) : Sodium phosphate, dibasic, Na2HPO4 (MM=142.0) pKa = 6.86 a) Choose the buffer system most effective at pH of 5.4 b) Calculate the amounts of weak acid and conjugate base you need to make 25mL of 0.25M solutions (one...