1.Show calculations for making following reagents A. 200 ml of a 1M solution of Tris (molecular...
1.Show calculations for making following reagents
A. 200 ml of a 1M solution of Tris (molecular weight 121.1 g/mol)
B. 300 ml of 0.2M EDTA (MW 372.2 g/mol)
C. 200 ML of TE buffer (contains 10 mM Tris and 1 mm EDTA) using solutions made in A and B
A solution containing a concentration of 0.01M (H+) ions will have a pH of ---
3. What is the Henderson Hasselbach equation? Use this equation to explain the relationship between pH and dissociation constants.
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1.Show calculations for making following reagents
A. 200 ml of a 1M solution of Tris (molecular...
1) Solution Components A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4 B...
1) Solution Components A 1 mL 100 mM NaH2PO4 + 9 mL 100 mM Na2HPO4 B 5 mL 100 mM NaH2PO4 + 5 mL 100 mM Na2HPO4 C 9 mL 100 mM NaH2PO4 + 1 mL 100 mM Na2HPO4 D 10 mL 100 mM NaH2PO4 stock solution E 10 mL 100 mM Na2HPO4 stock solution F 10 mL distilled water pKa of phosphate: 6.8 How would you calculate the pH of each equation using the Henderson Hasselbalch equations? 2) Tris...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric...
If you needed to make 500 mL of a TBE buffer (containing 0.1M Tris base, 0.1M Boric Acid, and 0.02M EDTA) how much of each of the following would you use: solid Tris base (121.1 g/mol); solid boric acid (61.8 g/mol); liquid stock solution of 0.50M EDTA?
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a)...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
1. Show your working out for the following calculation a) TENS buffer:  
1. Show your working out for the following calculation a) TENS buffer: Make from 0.1M NaOH from stock 10M 0.2% SDS from 10% stock 1mM EDTA from 0.5M stock 10mM Tris pH 7.5 from 1M stock How would you make 200ml of TENS buffer from these stock solutions? b) 5M LiCl How would you make 5M LiCl (MW=42.40g/mol)? c) 70% Ethanol How would you make 1L of 70% Ethanol? d) HisTag Protein Purification Solutions Charge Buffer 50mM NiSO4.6H2O Binding...
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; t...
Show calculations for the solutions.
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
9.30 mL of 0.1 M HCl are added to 200 mL of the buffer solution prepared...
9.30 mL of 0.1 M HCl are added to 200 mL of the buffer solution prepared in #8. What is the resulting pH? 8. A buffer solution is made by adding 20.4 grams of sodium acetate (NaCH CO2) toa 0.2M acetic acid (CH,CO2H) solution. The total volume of the resulting solution is exactly 1L. What is the phH of this solution? (Note: the pK, of acetic acid is 4.74) 20.4q NaCH3(02 82.034 9/mO 8203y g/mol .25 025 nol 1.0しー= 0.25mol...
can someone help with #2 150 mL of 10-1-(WA) SDS 1w/veg of solute/100 ml 10g: 100...
can
someone help with #2
150 mL of 10-1-(WA) SDS 1w/veg of solute/100 ml 10g: 100 ml 150 ml 10g) 15g SDS in 150 ml dd Haa (100ml) b. 200 mL of 250mm tris buffer, por 7.5 Tris 1210 g/mol PH 7.5 es pot - 14-7.5 = 6.5 Pka of tris: 205 -> pkb - 14-pka = 5.94 POH pkb + log [ BH"] Balt [B]-* [et] Tel : antilog (6.5-5.94) - 3.631 [4] . 3.631 [8] [BHT] + [b],...
Histidine can be treated as a polyprotic acid for the purpose of making a buffer solution....
Histidine can be treated as a polyprotic acid for the purpose of making a buffer solution. The molecular weight histidine is 155.2 g/mol. You are asked to make 200 mL of a buffer solution containing 50 mM L-histidine. How much histidine do you add to the solution? Show your work. You adjust the pH of this solution to pH 5.0. Please draw the structures of the two primary histidine species at this pH. Circle the structure that is most prevalent...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
are making 1 L of Ni-NTA Buffer (100 mM Tris, 200 mm NaCl, pH 7.8). a) Calculate the mass of NaCl (58.44 MW) to add to the buffer. b) Calculate the mass of Tris base (121.1 MW) to add to the buffer. c) You then measure the pH of the buffer and find it to be 8.2. What amount of 1 M HCl must be added to reach a pH of 7.8? pKa of Tris is 8.06. Ignore volume change...
Show calculations for the solutions.
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
9.30 mL of 0.1 M HCl are added to 200 mL of the buffer solution prepared in #8. What is the resulting pH? 8. A buffer solution is made by adding 20.4 grams of sodium acetate (NaCH CO2) toa 0.2M acetic acid (CH,CO2H) solution. The total volume of the resulting solution is exactly 1L. What is the phH of this solution? (Note: the pK, of acetic acid is 4.74) 20.4q NaCH3(02 82.034 9/mO 8203y g/mol .25 025 nol 1.0しー= 0.25mol...
can
someone help with #2
150 mL of 10-1-(WA) SDS 1w/veg of solute/100 ml 10g: 100 ml 150 ml 10g) 15g SDS in 150 ml dd Haa (100ml) b. 200 mL of 250mm tris buffer, por 7.5 Tris 1210 g/mol PH 7.5 es pot - 14-7.5 = 6.5 Pka of tris: 205 -> pkb - 14-pka = 5.94 POH pkb + log [ BH"] Balt [B]-* [et] Tel : antilog (6.5-5.94) - 3.631 [4] . 3.631 [8] [BHT] + [b],...
Histidine can be treated as a polyprotic acid for the purpose of making a buffer solution. The molecular weight histidine is 155.2 g/mol. You are asked to make 200 mL of a buffer solution containing 50 mM L-histidine. How much histidine do you add to the solution? Show your work. You adjust the pH of this solution to pH 5.0. Please draw the structures of the two primary histidine species at this pH. Circle the structure that is most prevalent...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
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