Incubation of RNA in high pH solutions results in degradation of the RNA molecule. Draw the...
Incubation of RNA in high pH solutions results in degradation of the RNA molecule. Draw the reaction mechanism of this alkaline hydrolysis process of RNA.
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Incubation of RNA in high pH solutions results in degradation of
the RNA molecule. Draw the...
Consider the reaction that causes RNA to hydrolyze at high PH. Why is RNA more sensitive...
Consider the reaction that causes RNA to hydrolyze at high PH. Why is RNA more sensitive to high PH than DNA? What is the function of Mg2+ in RNA molecule?
7. Why is RNA less stable than DNA? b) it is subject to hydrolysis at high...
7. Why is RNA less stable than DNA? b) it is subject to hydrolysis at high pH a) it is subject to hydrolysis at low pH it is subject to degradation by nucleases d) both a and c are reasons e) both b and c are reasons 8. Which of the following are NOT types of DNA damage? a) deamination b) depurination c) oxidative damage e) depyrimidination d) alkylation 9. What is the role of restriction endonucleases in molecular cloning?...
The hydrolysis of pNG results in the formation of one molecule of pNit. However, the hydrolysis...
The hydrolysis of pNG results in the formation of one molecule of pNit. However, the hydrolysis of cellobiose results in the formation of two glucose molecules. How would the measured rate of pNit “monomer” formation (reaction rate) appear if cellobiase produced two pNit “monomers” for each pNG molecule? Your answer for “a” is only an apparent rate. What would you have to do to your measured rates to calculate the actual rate of reaction?
You are analysing RNA transcripts of one particular gene in different tissues from mice. Using a...
You are analysing RNA transcripts of
one particular gene in different tissues from mice. Using a
specific probe to detect the RNA transcripts in embryonic heart
(EH), adult heart (AH), embryonic pituitary (EP), adult pituitary
(AP), and testis (T) the following pattern is observed:
What would you conclude about the gene
based on the results with AH RNA? Explain the possible underlying
molecular mechanism.
How would you explain the results with
testis RNA?
Explain the possible underlying
molecular mechanism. Which...
PART Ill: MEASURING PH OF VARIOUS SALT SOLUTIONS L Classify each salt as neutral, acidic or...
PART Ill: MEASURING PH OF VARIOUS SALT SOLUTIONS L Classify each salt as neutral, acidic or basic Provide an explanation based on measured pH and show the hydrolysis Salt NaCI ysis reaction to determine which ion hydrolyzes to Salt Type Measured pH Hydrolysis reaction NaCzH302 1.11 0.10 G.02 6.70 7.6 NaHCOs NazCO NHAC NH4C:H:02 il. Calculate Ki for NHaCl using measured pH: Assume that (NH ; 1 is equivalent to initial INH C. Ki for NH4Ch
Write the rate law for the reaction. Hydrolysis of PNA OH high pH +H2O HO NO2...
Write the rate law for the reaction.
Hydrolysis of PNA OH high pH +H2O HO NO2 NO2 -nitrophenylacetate (PNA) p-nitrophenol high pH p-nitrophenolate (PNP)
B. pH of Salt Solutions: Write net-ionic equations for the interaction of the ions in each...
B. pH of Salt Solutions: Write net-ionic equations for the interaction of the ions in each salt with water and explain why each salt is acidic, basic, or neutral as observed by the measured pH values. pH of Salt Solutions: B. Salt Solution: NaC,H,O, NH CI Na,CO, 6.90 6.13 Measured pH HOTTA Based on your measured pH values, indicate whether the aqueous solution of the salt is acidic, basic, or neutral. Write the net-ionic equations for any hydrolysis (reaction with...
B. pH of Salt Solutions: Salt Solution: NaC,H,O, Measured pH 6.90 NHC 6.13 Na.co, 10.78 Based...
B. pH of Salt Solutions: Salt Solution: NaC,H,O, Measured pH 6.90 NHC 6.13 Na.co, 10.78 Based on your measured pH values, indicate whether the aqueous solution of the salt is acidic, basic, or neutral. Write the net-ionic equations for any hydrolysis (reaction with water) that occurs. If no hydrolysis occurs, write "No Reaction. Explain in words why each salt is acidic, basic, or neutral. NaC,H,02 Nat +H,0 C,H,O,- + H,0 NH CI NH, +H04 Cl + H,0 Na,Co Nat +HO...
3. Draw the chemical mechanism (with electron-pushing arrows) for the first step of RNA splicing (as...
3. Draw the chemical mechanism (with electron-pushing arrows) for the first step of RNA splicing (as catalyzed by the splicosome). Be sure to explicitly show the attacking nucleophile, the phosphate at the intron-exon junction, the intron branch point and the leaving group in the reaction.
Draw the structure of the polymer (the repeating unit) that results when the following molecule is...
Draw the structure of the polymer (the repeating unit) that results when the following molecule is treated with peroxide (ROOR). 5. ROOR Polylethylene terephthalate) can be synthesized from terephthalic acid and ethylene Blycol in the presence of an acid catalyst and heat. 6. acid catalyst heat ??-?- (a) What are the two products of the reaction? (b) What is the acronym of Poly(ethylene terephthalate)?
7. Why is RNA less stable than DNA? b) it is subject to hydrolysis at high pH a) it is subject to hydrolysis at low pH it is subject to degradation by nucleases d) both a and c are reasons e) both b and c are reasons 8. Which of the following are NOT types of DNA damage? a) deamination b) depurination c) oxidative damage e) depyrimidination d) alkylation 9. What is the role of restriction endonucleases in molecular cloning?...
You are analysing RNA transcripts of
one particular gene in different tissues from mice. Using a
specific probe to detect the RNA transcripts in embryonic heart
(EH), adult heart (AH), embryonic pituitary (EP), adult pituitary
(AP), and testis (T) the following pattern is observed:
What would you conclude about the gene
based on the results with AH RNA? Explain the possible underlying
molecular mechanism.
How would you explain the results with
testis RNA?
Explain the possible underlying
molecular mechanism. Which...
PART Ill: MEASURING PH OF VARIOUS SALT SOLUTIONS L Classify each salt as neutral, acidic or basic Provide an explanation based on measured pH and show the hydrolysis Salt NaCI ysis reaction to determine which ion hydrolyzes to Salt Type Measured pH Hydrolysis reaction NaCzH302 1.11 0.10 G.02 6.70 7.6 NaHCOs NazCO NHAC NH4C:H:02 il. Calculate Ki for NHaCl using measured pH: Assume that (NH ; 1 is equivalent to initial INH C. Ki for NH4Ch
Write the rate law for the reaction.
Hydrolysis of PNA OH high pH +H2O HO NO2 NO2 -nitrophenylacetate (PNA) p-nitrophenol high pH p-nitrophenolate (PNP)
B. pH of Salt Solutions: Write net-ionic equations for the interaction of the ions in each salt with water and explain why each salt is acidic, basic, or neutral as observed by the measured pH values. pH of Salt Solutions: B. Salt Solution: NaC,H,O, NH CI Na,CO, 6.90 6.13 Measured pH HOTTA Based on your measured pH values, indicate whether the aqueous solution of the salt is acidic, basic, or neutral. Write the net-ionic equations for any hydrolysis (reaction with...
B. pH of Salt Solutions: Salt Solution: NaC,H,O, Measured pH 6.90 NHC 6.13 Na.co, 10.78 Based on your measured pH values, indicate whether the aqueous solution of the salt is acidic, basic, or neutral. Write the net-ionic equations for any hydrolysis (reaction with water) that occurs. If no hydrolysis occurs, write "No Reaction. Explain in words why each salt is acidic, basic, or neutral. NaC,H,02 Nat +H,0 C,H,O,- + H,0 NH CI NH, +H04 Cl + H,0 Na,Co Nat +HO...
3. Draw the chemical mechanism (with electron-pushing arrows) for the first step of RNA splicing (as catalyzed by the splicosome). Be sure to explicitly show the attacking nucleophile, the phosphate at the intron-exon junction, the intron branch point and the leaving group in the reaction.
Draw the structure of the polymer (the repeating unit) that results when the following molecule is treated with peroxide (ROOR). 5. ROOR Polylethylene terephthalate) can be synthesized from terephthalic acid and ethylene Blycol in the presence of an acid catalyst and heat. 6. acid catalyst heat ??-?- (a) What are the two products of the reaction? (b) What is the acronym of Poly(ethylene terephthalate)?
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