Glucose labeled with 14C atom in position C-1 is incubated with glycolysis enzymes. what part (%) of...
Glucose labeled with 14C atom in position C-1 is incubated with glycolysis enzymes. what part (%) of pyruvate molecules will be labeled
Homework Answers
One glucose molecule catabolised into two pyruvate molecules through 10 step reaction process known as Glycolysis.
Glucose is a Six Carbon molecule and Pyruvate is a 3 carbon molecule.
In the 4th step of Glycolysis ( when Fructose-1,6-bisphosphate to Dihydroxy acetone phosphate and Glyceralde-3-phosphate ) the six carbon atom chain break into two 3-carbon atom molecule.
One pyruvate molecule will have carbon atoms C1,C2 and C3 and other will have Carbon atoms C4,C5 and C6.
So, 50% of pyruvate molecules will have labeled carbon isotope 14C.
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Glucose labeled with 14C atom in position
C-1 is incubated with glycolysis enzymes. what part (%) of...
If a molecule of glucose is labeled with 14C at position 1 proceeds through glycolysis, where...
If a molecule of glucose is labeled with 14C at position 1 proceeds through glycolysis, where will the radioactive label end up? a) At the carboxyl carbon of all the pyruvate produced b) At the carboxyl carbon of half of the pyruvate produced. c) At the methyl carbon of half of the pyruvate produced. d) At the methyl carbon of all the pyruvate produced. e) At the carbonyl carbon of all the pyruvate produced. f) At the carbonyl carbon of...
Drawing Glycolytic intermediates. Glucose labeled with 14_C at C-1 was incubated with the glycolytic enzymes and...
Drawing Glycolytic intermediates. Glucose labeled with 14_C at C-1 was incubated with the glycolytic enzymes and necessary cofactors. Draw structures to show the transformation of glucose to two molecules of glyceraldehyde 3-phosphate via the first half of glycolysis, clearly indicating the position of the 14_C label at each step. You do not need to show reaction mechanisms for each reaction.
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled...
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled in: A) its methyl carbon B) all three carbons C) its carbonyl carbon D) its carboxyl carbon E) A and D
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex. What percentage of the acetyl-CoA molecules formed will be labeled with 14C, and in which position of the acetyl moiety will the 14C label be found? Select the correct answer and explain your reasoning 100% of the acetyl-CoA will be labeled at C-1 (carboxyl). 100% of the acetyl-CoA will be labeled at C-2. 50% of the acetyl-CoA will...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex. What percentage of the acetyl-CoA molecules formed will be labeled with 14C, and in which position of the acetyl moiety will the 14C label be found? Select the correct answer and explain your reasoning 100% of the acetyl-CoA will be labeled at C-1 (carboxyl). 100% of the acetyl-CoA will be labeled at C-2. 50% of the acetyl-CoA will...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex. What percentage of the acetyl-CoA molecules formed will be labeled with 14C, and in which position of the acetyl moiety will the 14C label be found? Select the correct answer and explain your reasoning 100% of the acetyl-CoA will be labeled at C-1 (carboxyl). 100% of the acetyl-CoA will be labeled at C-2. 50% of the acetyl-CoA will...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via...
Question 3 Glucose labeled with 14C in C-3 and C-4 is completely converted to acetyl-CoA via glycolysis and the pyruvate dehydrogenase complex. What percentage of the acetyl-CoA molecules formed will be labeled with 14C, and in which position of the acetyl moiety will the 14C label be found? Select the correct answer and explain your reasoning 100% of the acetyl-CoA will be labeled at C-1 (carboxyl). 100% of the acetyl-CoA will be labeled at C-2. 50% of the acetyl-CoA will...
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liv...
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast cell extract just long enough that each intermediate in the ethanol fermentation is labeled with a 14C (pulse). The 14C label is then diluted so that no additional radioactivity is incorporated into the intermediates (chase). 1. What position in ethanol will be labeled with 14C if 14C-glucose labeled at position C-1 is used? 2. Where should the 14C label sit in glucose for it...
Answer any one and only one of the following: a. If glucose labeled with 14C in...
Answer any one and only one of the following: a. If glucose labeled with 14C in C-1 were fed to yeast cells carrying out anaerobic metabolism, what carbon atom would be tagged with 14C in what compound? b.If palmitic acid (16:0) labeled in C-1 were metabolized under anaerobic metabolism in mammalian tissues, what carbon atom would be tagged with 14C in what compound?
Drawing Glycolytic intermediates. Glucose labeled with 14_C at C-1 was incubated with the glycolytic enzymes and necessary cofactors. Draw structures to show the transformation of glucose to two molecules of glyceraldehyde 3-phosphate via the first half of glycolysis, clearly indicating the position of the 14_C label at each step. You do not need to show reaction mechanisms for each reaction.
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled in: A) its methyl carbon B) all three carbons C) its carbonyl carbon D) its carboxyl carbon E) A and D
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
Answer any one and only one of the following: a. If glucose labeled with 14C in C-1 were fed to yeast cells carrying out anaerobic metabolism, what carbon atom would be tagged with 14C in what compound? b.If palmitic acid (16:0) labeled in C-1 were metabolized under anaerobic metabolism in mammalian tissues, what carbon atom would be tagged with 14C in what compound?
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