What is the general principle behind using an autoclave? How does it work? What is the...
- What is the general principle behind using an autoclave? How does it work?
- What is the advantage of using an autoclave (generates steam with high pressure and temperature) to sterilize surgical instruments rather than simply boiling them in water?
- Why must containers of liquid be open prior to autoclaving?
- What are sterility indicators? Why must they be used?
Homework Answers
Autoclave uses the sterilization principle by the steam sterilization process as it contains the latent heat inside it; hence it exerts the heavy pressure and show high penetration in the microbial cells imparting efficient destruction. (Advantage rather than boiling in water)
It is normally operated at 121 0C temperature and 15 PSIG pressure. .
The containers of liquid are open prior to autoclaving as there is chances of the busting due to the increase in pressure in the container while autoclaving.
The sterility indicators are the biological or physical indicators which are used to check whether the sterilization equipment is working properly or not means for validation of the sterilization equipments.
Eg: Browney’s Heat tube in validation of Hot air oven
Bacillus stearothermophilus for the autoclave validation
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