Question

A group of analysts was working on developing a method to separate and determine carbamate insecticides used for control of pests in food production They used HPLC with an Eurospher 100-5 C-18 column and a mobile phase consisting of a mixture of water and methanol, with post-column derivatization. .The column was determined to have an average of 1999 theoretical plates for this group of compounds. The stationary and mobile phases volumes in the column were SMV = 0.51mL, V = 1.42mL, respectively, and the mobile phase flow rate was 0.43mL/min. If the partition coefficients for two of the insecticides compounds A (Propoxur) A and B (Carbofuran) were K_A = 6.20 and K_B = 7.15, respectively, and t_M = 1.20 minutes, calculate the following:

Show all work please

a)capacity factors, k’_A and k’_B

b)selectivity factor,a

c)retention times, t_RA and t_RB

d)peak widths, w_A and w_B

e)resolution, R_s. Is base line resolution achieved?

Answer 1

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(c) Retention tince stand to tm = 2.22=tA - 1.20 1.20 = 3.86 miles) tui WA 2.562 t B - 1.20 1.20 thee 4.27 nenules/ (o) width of peaks calculation Na 16 to ² N = 10 (6) 2 1999- 1613.86) 1999 = 18 (4:27) N (11. 177)= 3.86 11.177) = 4.27 "IWA= 0.345 WB=0.382 ce) Resolution Rs = 2 ( t = 2(4.27 - 3.86) WA WR 0.312 + 0.345 | Resolution 1.12

o According to data fovided t o Theoretical plates in column= 1999 (AN) Volume of column in stationary phase = 0.51 me Volume of mobile phrase = 1.42me Mobile phase flow rate = 0. 43 ml ſnin. Partition coefficient of A (KA) = 6.20 B (kB) - 7.15 t = 1,20 minutes (a) Capacity factor (kn & kB) & k = KA (VS) = 6.20 (0.51 ) me 1.42 /nd 1.42 ker' = Ro lie) = 7.15(043) (Capacity factors) = 2.56 Retention thrine of solute A is 2.22 and B is 2.56 no (6) Selectivity facler a = RA = 222 ko 2,56 x = 0.86 IN V