Question

biochem

Enolase catalyzes the two-step B-elimination of water from 2-phosphoglycerate to generate 5. phosphoenolpyruvate. The enzyme provides a protonated Glu, a deprotonated Lys, and a Mn2 ion to catalyze the reaction. Draw the mechanism for this reaction. OPO32 OPO32 enolase OH

Answer 1

Mechanism Enolay Enolase Enease Mezt Ma2t Enolase Mg 24 и,о -OPO + CH2 OPO3 ERetage NH CH C Hh o-H C =O H-O CH2 CH2 CHI CH2 CH2 CH2 Eno eaLus Gl 2Enolase GlEnolase anionic intermedate) The en3yme d enolase bind do dh carbony group the 2 phosphoglarate dhveugh tuwo Manesium ions ohich on ha deprotonated ogen and slablizes h negadive change makss dh alpha hydrogen o 2 phospho yceratemove aciolic. Lys alaprodona des dhe o-Hgelrogen rom 2phosphoslyearates usul in he ovmadion of Enolars 345 a Car banion inbermediate cohich was and stabilised b dh M ion of dhe carbory late o the en3mss adom The hydrogida group on dhu pCadom is eliminated as waber dua do dhe eacdon sidh re sonance GlEnolare esults In da madhon o phosphoenol rmade