If glucose were radiolabeled with 14C in C-2, what carbon would you find the radiolabel in...
If glucose were radiolabeled with 14C in C-2, what carbon would
you find the radiolabel
in phosphoenolpyruvate?
a. 1
b. 2
c. 3
d. all carbons
e. cannot be determined
please provide the mechanism
Homework Answers
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If glucose were radiolabeled with 14C in C-2, what carbon would
you find the radiolabel
in...
If the C-2 carbon of glucose were labeled with 14C, which if the carbons atoms in...
If the C-2 carbon of glucose were labeled with 14C, which if the carbons atoms in 3PG would be labeled during glycosis ? Give a numeric answer please .
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate...
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate formed would be labeled in (pick from list given below) i) all three carbons ii) its carbonyl carbon iii) its carboxyl carbon iv) its methyl carbon Ali B) ii C) iii D) iv E) A and C
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled...
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled using O. Please draw out the pathway showing where this radiolabel is in each step of the pathway, b. In glycolysis, ATP molecules are produced. Please write out the reactions that utilize ATP as well as the ones that create ATP, and explain how many molecules of ATP are made in glycolysis. c. A reaction is set up with 3-phosphoglycerate, where the phosphate atom...
1. You have acetate that is isotopically labelled (13C) at carbon 1 and radiolabeled (14C) at carbon...
1. You have acetate that is isotopically labelled
(13C) at carbon 1 and radiolabeled (14C) at
carbon 2. Assume all the acetate gets incorporated into
Acetyl-CoA. Calculate the % of each labelled carbon that
gets released in the first five rounds of the TCA cycle and at what
enzymatic step they get released.
2. Document (on extra pages attached to this sheet)
your work. Note: you don’t have to include
every step if you are clever.
1. You have acetate that is isotopically labelled (c) at...
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled...
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled in: A) its methyl carbon B) all three carbons C) its carbonyl carbon D) its carboxyl carbon E) A and D
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liv...
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
The complex metabolic pathways in the parasite Trypanosoma brucei were elucidated in part by adding radiolabeled...
The complex metabolic pathways in the parasite Trypanosoma brucei were elucidated in part by adding radiolabeled metabolites to cultured parasites. In the parasite, glucose is converted t0 phosphoenolpyruvate (PEP) in the cytosol. PEP then enters an organelle called the glycosome and is converted to oxaloacetate (OAA); OAA is then converted to malate, and malate to fumarate. Fumarate reductase catalyzes the conversion of fumarate to succinate; the succinate is then secreted from the glycosome. If C1 of glucose is labeled, what...
Many metabolic pathways were mapped using radiolabels in a pulse-chase experiment. A pulse of radiolabeled substrate...
Many metabolic pathways were mapped using radiolabels in a pulse-chase experiment. A pulse of radiolabeled substrate would be added to growing cells (so that it enters the metabolic pathway) followed by a chase of unlabeled substrate to push the label through the pathway and into the products. If a cell is fed 14C-glucose (14C is on carbon 4), where does the 14C end up a. in 3-phosphoglycerate? (indicate on the structure) b. in pyruvate? c. after the pyruvate dehydrogenase reaction?
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the...
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the 14C label ends up in compound C during steady-state metabolism in liver. COO- çoo- FO +H COO- H *CH3 a. What is the metabolic pathway involved? Write the reaction including all reactants and products, no structures required (1) b. Which carbons in compound C will become labeled, and to what percent? Briefly explain. (3) c. What is the physiological basis and the molecular mechanism...
9. You are given Ribulose-5-Phosphate, with a 14C label carbon in the position-2 and position- 3....
9. You are given Ribulose-5-Phosphate, with a 14C label carbon in the position-2 and position- 3. Given the pathway below, draw the numbered (1 to 5) structure(s) and provide the correct names AND illustrating the retention and position(s) of the radiolabel carbon(s) with the resulting carbohydrate intermediates (7 marks) ® 5C 7C 6C — 6C 5C 3C 4 0 6C 5C 30 -
If glucose labeled with 14C at C-2 were metabolized in the liver, the first radioactive pyruvate formed would be labeled in (pick from list given below) i) all three carbons ii) its carbonyl carbon iii) its carboxyl carbon iv) its methyl carbon Ali B) ii C) iii D) iv E) A and C
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled using O. Please draw out the pathway showing where this radiolabel is in each step of the pathway, b. In glycolysis, ATP molecules are produced. Please write out the reactions that utilize ATP as well as the ones that create ATP, and explain how many molecules of ATP are made in glycolysis. c. A reaction is set up with 3-phosphoglycerate, where the phosphate atom...
1. You have acetate that is isotopically labelled
(13C) at carbon 1 and radiolabeled (14C) at
carbon 2. Assume all the acetate gets incorporated into
Acetyl-CoA. Calculate the % of each labelled carbon that
gets released in the first five rounds of the TCA cycle and at what
enzymatic step they get released.
2. Document (on extra pages attached to this sheet)
your work. Note: you don’t have to include
every step if you are clever.
1. You have acetate that is isotopically labelled (c) at...
21. Glucose labeled with 14C in C-1 and C-4 gives rise in glycolysis to pyruvate labeled in: A) its methyl carbon B) all three carbons C) its carbonyl carbon D) its carboxyl carbon E) A and D
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated in a cell-free liver homogenate under anaerobic conditions, which carbon in the lactate produced would be labeled with 1℃ (refer to the carboxyl carbon of lactate as C1, the central carbon as C2, and the methyl carbon as C3)? What if theglucose were labeled at carbon #3 instead? In both cases, explain your answer.
.) (3pts) If radioactive glucose labeled at carbon #6 with 14C, were incubated...
Many metabolic pathways were mapped using radiolabels in a pulse-chase experiment. A pulse of radiolabeled substrate would be added to growing cells (so that it enters the metabolic pathway) followed by a chase of unlabeled substrate to push the label through the pathway and into the products. If a cell is fed 14C-glucose (14C is on carbon 4), where does the 14C end up a. in 3-phosphoglycerate? (indicate on the structure) b. in pyruvate? c. after the pyruvate dehydrogenase reaction?
Q4. Compound A is labeled at the methyl carbon with 14C (*). Under fasting conditions, the 14C label ends up in compound C during steady-state metabolism in liver. COO- çoo- FO +H COO- H *CH3 a. What is the metabolic pathway involved? Write the reaction including all reactants and products, no structures required (1) b. Which carbons in compound C will become labeled, and to what percent? Briefly explain. (3) c. What is the physiological basis and the molecular mechanism...
9. You are given Ribulose-5-Phosphate, with a 14C label carbon in the position-2 and position- 3. Given the pathway below, draw the numbered (1 to 5) structure(s) and provide the correct names AND illustrating the retention and position(s) of the radiolabel carbon(s) with the resulting carbohydrate intermediates (7 marks) ® 5C 7C 6C — 6C 5C 3C 4 0 6C 5C 30 -
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