Question

Exercise 3: Rapid Chromatographic immunoassay for Human Chorionic Gonadotropin (hCG) in Serum or Urine Purpose The student will perform a qualitative test for pregnancy using a rapid chromatographic immunoassay test methodology for the detection of Human Chorionic Gonadotropin (hCG). Human chorionic gonadotropin (hCG) hCG is a glucoprotein hormone comprised of two subunits: alpha and beta (B). The alpha subunit is similar in amino acid sequence to other hormones such as FSH, LH and TSH. The B subunit is not similar in structure and therefore does not cross react to give false positive results. Detection of the B subunit is a specific indicator of hCG. Indications for testing • Pregnancy (not present in males or non-pregnant women) • May be elevated in: • neoplasms such as hydatidiform, mole, choriocarcinoma • cancers such as testicular, breast, hepatoma, small intestine, lung Before you begin • Bring all reagents to room temperature • Mix each liquid reagent by gentile agitation or swirling • Label each cylinder with appropriate specimen identification • Use a separate plastic transfer pipette for each specimen or control Questions 1. Briefly discuss and diagram the principle of the chromatographic immunoassay used. 2. List three possible sources of error affecting the procedure leading to false-positive or false-negative results. 3. What would you suggest be done if a patient's specimen was classified as not interpretable.

Answer 1

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