Question

In this experiment, you will carry out ALP analyses of two simulated blood serum samples: a "normal" (in terms of ALP level) serum sample, and a hypothetical patient serum sample. You will determine the amount of ALP in each sample, using kinetic enzyme assays and graphing techniques. You will then draw conclusions about possible bone or liver disease in the hypothetical patient.

Answer 1

16 MONDAY 9 10 11 12 13 14 15 16 17 18 19 20 21 29 23 24 25 26 27 28 29 30 31 JANUARY MONDAY Alkaline Phosphate CALP) What do the test result means High levels - Higher than a normal levels of ALP in 11 your blood may indicate a problem with your liver or gall bladder. This could include hepatitis cirhosis, liver cancer, gallstones, or a blockage in your bile ducts 1 High levels may also indicale an issuce related to the bonee such as rickets, Paget's disearr i bone cancer, or an overachu 3 para thyroid gland. In rare cases high ALP level can indicate heart failure kidney cancer, other cancer, s mononucleosis or bacterial infections, • Low levels Having lower - Shan-nonnal Ace levels in your blood is rove, but it can indicate maluution, which could be caused by celiac disease or a deficiency in certain vitamins and minerals.

TUESDAY Conclusion Blood levels of ALP also increase by two to four timer during pregnancy. This is a result of additional alkaline phosphatase produced by the placenta. Additionally, abnommal level of ALP In the blood could indicate issue relating to the liver, gall bladder or bones. - There the hypothetical patients ALP value is fous times increase that of a normal person. So if the hypothetical patient is pregnant then it is seem to be honal or chaneer of problems include lover, gallbladder, problem related to bones etc. are common January DECEMBER S M T W T 1 15 29 F S 2 3 16 17 30 31 S 4 18 2011 M T W T F S 5 6 7 8 9 10 19 20 21 22 23 24 Fri Week 02 013-353 11 12 25 26 13 14 27 28 2012 A supid quantitative assay foe measueing alkaline phosphatase activity in osteoblastic cells in vitro ... Dikaline phosphertese is the most widely recognized biochemical marked for osteoblast aldivity. Although its precise sunetion is poorly understood It is believed to play a role in skeletal mineralization. Scanned with CamScanner

ALP (alkaline Phosphatuse Level] An alkaline Phosphatuse level test measures the amonnt of alkaline Phosphatase enzyme in Blood Stream The test requires a simple blood dean and is often a routine past of other Blood tests. The noemal range of ALP varies beom peason to peeson and depends on age, blood type gendes, and whether pregnant. The Nolmal range for Selum, ALP level is 20 to 140 10/L The lives is one of the main i sources of ALD, But some is also made in bones intestines, pancreas, and kidneys. in Peeghata women ALP is made in the placenta The ALP test can be helpful in idendibying Conditions such as: • hepatitis ocirehosis a cholecystitis • Blockage of bile ducts.

26 27 Week 02.012-354 2012 Wed 2012 Week 2011-355 The ALP test can be helppulin the diagnosis of bone peoblems such as • Rickets . Osteomalacia • Paget's disease of the bone ALP testing may also be helpful in investigating the peesence of Canced, tumors, unusual bone ! geowth, ol vitamin D deficienly Id can also be used to check the peogless of treatment for any of the above condition. Low levels of alkaline phosphalos may indicale hypophosphatasia a sare genetic disease that appeets bones and teeth. Low levels may also be due to a deficiency of zinc ox malnutrition Having lower than nosmal ALP levels in blood is sare, but it can indicale malnutrition, which could be cansed by celice disease of a deficiency in Certain Vitamins conch minesals. Abnoemal levels of ALP in blood most often indicate a problem with lives, gallbladdee, ol bones However they may on also indicale malnutrition, kidner Can les tumoes intestinal issues, a pancreas Problem on a serious infection! High DLP usually means that either the lived has been damaged os a lond fon causing increased bone rall activita is keesent. Todal Alkaline Phosphatase - Noemal Range : 40- 130 U/L Livee fraction : 6920/2 Bone flaetion : azul intestinal reaction : 18 u