Question

Calculation of Drug at 5 Doses for Administration bid for 3 Days to Mice Example method for Drug 1 (95% free base) to be mixed once to be administered via any route bid for 3 days to mice. Dose levels 64,32,16,8,4& mg/kg/day. Drug 1 64(32 bid) Mix 104.4ng of Drug 1 in mg in 31 ml HEC Grind in 1 mortar and make dilutions as follows: 04.0 64mg A Mix 104.4 mg in 31ml HEC 16ml + 15ml 31ml ns (15ml of A+15ml of HEC) 32meB 16ml + 14ml = 30ml 16mgC (14ml of B+ 14ml of HEC 16 ml+12ml 28ml 8mgb 12ml of c+12ml of HEC 16ml +8ml 24ml 4mgE (8ml of D+ 8ml of HEC 16ml To figure how much drug to weigh out once for the preparation of all 5 dose levels use this formula: 31ml HEC 32mg/10/0.95 014 mg to weigh out for the 5 dose preparation 10 is a number someone determined needed to be used so the volume of drug given to a mouse could be simply the amount in mls according to the weight of the mouse. If the mouse weighs 25g then 0.25ml of the dose would be given bid for 3 days. I don't know what this number would be for another animal or man.

Using the example on the first sheet, figure out how much drug at each dose (second sheet) for administration bid( twice daily)for 3 days to mice.

The Free base of the drug is 91.62% and the vial =16 ml.

Answer 1

Vial is 16ml

Free base % 91.62

Frequency BID

Formula used 31MEC×32mg/10/0.95=104.4mg

A. 256

B.128

C.64

D.32

E.8