Question

β galactosidase Activity (10 points) RESULS Ex. 1 1 a: Acoo | A550 Inc. Time | A420 Sample Vol Start Stop (ml) 0.1 O.0340.552 : 0 84 | :019 | .552 -162 O12 0.568 659.02568 03 .012 163 . 059 .01563 . 686022166 364 | : 013 | .166 。040| .Ol6 | .306 20:25 20:00 0.5 2 3 4 20s 0.1 40s 0.5 1 min 0.1 0.5 0.12 min C:3 6:07 1min 40s 21:51 6: 44 0.5 2 min 40s 22.51 2311 1 min 20s1 2l:3 6 0.5 2 min 20s 5:37 10 0.5 3 min Please

Calculate the specific activity for each sample using the formula:

1 unit (specific activity) = 1000 x (A₄₂₀– (1.75 x A₅₅₀) /   (T x V x A₆₀₀)

                                                          

where:

A₄₂₀= absorbance of the yellow ONP

A₅₅₀= cell debris light scattering

T = reaction time in minutes

V = volume of culture assayed (ml)

A₆₀₀= cell density

Sample	Calculation	Activity
1
2
3
4
5
6
7
8
9
10

Answer 1

Answers

Sample 1 - 33.86

Sample 2 - 83.97

Sample 3 -161.99

Sample 4 - 568.98

Sample 5 - 30.99.

Sample 6 - 58.99

Sample 7 - 85.99

Sample 8 - 363.99

Sample 9 - 39.99

Sample 10 - 42.99

Note: ( seconds convert to mints).

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