Question

1. Parameters measured in the use of flow cytometry for the diagnosis of hematologic malignancies include all of the following except A. Forward angle light scatter B. Right angle light scatter C. Bound antibody surface fluorescence D. None of the above 2. Forward angle light scatter is proportional to: A.Cell size B. Nuclear/cytoplasmic complexity C. Both 3. The purpose of the fluidics channel is used to: A. Allow detector measurement of scatter B. Allows cells to pass through laser one cell at a time C. Allows Fluorophores to emit light D. All of the above 4. The purpose of optics are to: A. Convert signals to usable data B. To sense the light C. To gather the light

Answer 1

1.The flow cytometry is used to assess and measure the cells.When the laser light assess the cell ,when it passes directly onnthe cell it is forward angle light scatter,the light which lyes at the sides or around the cell is called as Right angle light scatter or 90° light scatter. In flow cytometry the forward angle light scatter technique is used

Ans:A.Forward angle light scatter

2.The forward angle light scatter measures the dimension of a cell when observed.It measures it size.The side scatter light measures the nuclear complexity of a cell

Ans:A.Cell size

3.The main purpose of the fluidic system is to allow visualization of one cell at a time. It helps in clear picturisation of the cells and It a components

Ans:CB.Allows cells to pass through laser one at a time

4.The main purple of the optics in the flow metry is to gather or collect the light and enable the visualization of the cells effectively

Ans:C.To gather the light