Ans) Diagnosing sepsis can be difficult. The criteria for diagnosis include high or low body temperature, fast heart rate and respiratory rate, plus a probable or known infection.
There is no single test that can identify sepsis. Healthcare practitioners typically order a combination of tests to help diagnose sepsis, distinguish it from other conditions, detect the inflammation associated with sepsis, and to evaluate and monitor the function of the affected person's organs, blood oxygenation, and acid-base balance.
Laboratory Tests:
Some tests that may be done to detect sepsis and identify an
infection include:
Procalcitonin – may be used in conjunction with clinical
evaluations and other tests to determine the risk that seriously
ill patients will develop sepsis, or their risk of progressing to
severe sepsis, septic shock and risk of dying. This test can help
distinguish sepsis from other conditions that cause similar
symptoms and can help guide treatment. The level of procalcitonin
in the blood increases rapidly and significantly when a person has
sepsis.
Gram stains and cultures – these tests are done in conjunction with
one another. Cultures grow the bacteria that may be in a sample
taken from the site of suspected infection, so they can be
precisely identified and their susceptibility to various
antibiotics determined. Gram stains are used to detect the presence
and identify the general type of bacteria. A Gram stain may be done
relatively quickly but is not definitive—it provides only
presumptive results.
Blood culture – one type of culture used to detect bacteria in the
blood and evaluate their susceptibility to antibiotics
Urinalysis and urine culture – to identify whether the source of
infection is a urinary tract infection
Cerebrospinal fluid (CSF) analysis, including a culture – may
sometimes be done if it is thought that the person has an infection
of the central nervous system (meningitis or encephalitis).
Sputum culture – to help diagnose bacterial pneumonia
Cultures of other body fluids may be done as needed to detect the
source and type of infection.
Some general, non-specific tests that may be done include:
Complete blood count (CBC) – to evaluate red and white blood
cells and platelets
Lactate – increased levels can indicate organ dysfunction
Blood gases – to evaluate oxygen in the blood and acid-base
balance
Comprehensive metabolic panel (CMP) – to monitor the health of
organs, such as the kidneys and lungs, and monitor electrolyte
balance and blood glucose level
Prothrombin time (PT) and/or partial thromboplastin time (PTT) –
these tests may be done along with other clotting tests to help
evaluate the person's coagulation system—the process that the body
uses to form blood clots and stop bleeding.
C-reactive protein (CRP) – to detect inflammation in the body
Other tests may also be done as needed to help evaluate health
status or to identify or rule out complications or other
conditions, such as troponin to detect a heart attack.
Non-Laboratory Tests
These tests may be ordered to evaluate the health of organs, detect
complications, and to help locate an infection:
ECG – to evaluate heart rhythm or injury
X-ray
CT (computed tomography) scan
MRI (magnetic resonance imaging)
Ultrasound
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