Question

1. You are developing bioreactors for cardiac tissue engineering. a) One design is to apply mechanical stimulation. What kind of mechanical loading would you apply? b) Your colleague suggests using electrical stimulation instead of mechanical stimulation. Do you agree? Why? c) In both designs, what would be the advantages of a providing perfusion function to the bioreactor? a) Multivaniable optimization b) I agoll coase using mechanical stimulation will be hard on Cardiac lichile, but electrical is easier as you are dealing with cardiaic . C), der fusion medium through pores of scaffold & limprove cell wahility proliferation differentication & matrix synthesis

Answer 1

a) pulsatile flow and shear stress.

b) yes, I agree. Electrical stimulation shows increased intercellular communication and cardiomyocytes maturity, thus action potential of myocardium increases.

c) pre vascularization of cardiac tissues in vitro and to meet the demand of oxygen and nutrient to the cultured cells.