Carbonic anhydrase has two potential substrates: CO2 and HCO3 - . The KM value for CO2...
Carbonic anhydrase has two potential substrates: CO2 and HCO3 - . The KM value for CO2 is 12 mM and the KM for HCO3 - is 26 mM. Which substrate does carbonic anhydrase have a higher affinity for?
Homework Answers
Ans. Km, Michaelis-Menten constant, is defined as the [S] at which half-Vmax is attained. A lower value of Km indicates that the enzyme requires a lower [S] to reach its Vmax; in turn, it also indicates that a lower Km value exhibits higher affinity of the substrate for the enzyme.
Therefore, the substrate CO2 with lower Km value has a higher affinity for the enzyme.
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Carbonic anhydrase has two potential substrates: CO2 and HCO3 -
. The KM value for CO2...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the...
The enzyme carbonic anhydrase catalyzes the reaction CO2(g)+H2O(l)−→−HCO3−(aq)+H+(aq).CO2(g)+H2O(l)→ HCO3−(aq)+H+(aq). In water, without the enzyme, the reaction...
The enzyme carbonic anhydrase catalyzes the reaction CO2(g)+H2O(l)−→−HCO3−(aq)+H+(aq).CO2(g)+H2O(l)→ HCO3−(aq)+H+(aq). In water, without the enzyme, the reaction proceeds with a rate constant of 0.039s−10.039 s−1 at 25°C.25 °C. In the presence of the enzyme in water, the reaction proceeds with a rate constant of 1.0×106s−11.0×106 s−1 at 25°C.25 °C. Assuming the collision factor is the same for both situations, calculate the difference in activation energies for the uncatalyzed versus enzyme- catalyzed reaction.
IAt p6, carbonic anhydrase has a keue of 60,000s'. please estimate the ku, if the phf is 13 incre...
iAt p6, carbonic anhydrase has a keue of 60,000s'. please estimate the ku, if the phf is 13 increased to 7.0. Please justify your answer. Co,+ H20H2CO 14. The two person have the same k after drinking alcohol. Please calculate the substrate earbonie enhydrase concentration in Person 1. Please justify your answer. Person 1 Aldehyde koolS') Km (M) erson 2 dehydrogenase 0.0002 0.006 0.002 15. There is a tyrosine protease, which uses similar mechanism to a cysteine protease. Write down...
The following question focuses on how the parameters regulating enzyme function might change, and how these...
The following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menton plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20 > H+ + HCO3 There are many different isoforms of this enzyme. see for instance: http://en.wikipedia.org/wiki/Carbonic_anhydrase 1 Assume that one variant has a Km of 1 µM and a different variant has a Km of 10 µM....
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
help with these please The turnover number is defined as the maximum number of substrate molecules...
help with these please
The turnover number is defined as the maximum number of substrate molecules that can be converted into product molecules per unit time by an enzyme molecule. The concentration of enzyme active sites is not necessarily equal to the concentration of enzyme molecules, because some enzyme molecules have more than one active site. If the enzyme molecule has one active site, the turnover number is given by turnover number = - -=k2 (Rmax is often written as...
con condition o ste is half its maximal val 9. The Difference between Cellulose and Starch...
con condition o ste is half its maximal val 9. The Difference between Cellulose and Starch is A Starch doesn't Branch while Cellulose does. Starch contains alpha 16 pycosidic bonds, while Cellulose has beta 1,6 glycosidic bonds. C Starch is made up of carbohydrates and proteins, while cellulose is all carbohydrates Starch is in animals, while Cellulose is in plants. There is no difference between the two 10). An Enzyme catalyzes two different substrates, A and B. The Km for...
3. The enzyme that catalyzes the hydration of CO2 to H2CO3 has a Km = 12...
3. The enzyme that catalyzes the hydration of CO2 to H2CO3 has a Km = 12 mM and a initial speed of 4.5 micromol formed of H2CO3 / mL per second when [CO2] = 36 mM. a) What is vmax for this enzyme? b) Assuming that 5 pmol / mL (5 x 10-12 mol / mL) of the enzyme was used in the experiment, what is kcat for the enzyme? c) What is the catalytic efficiency of the enzyme? d)...
DI LUS his tell you about the T U SHIP Between substrate) and enzymes with high...
DI LUS his tell you about the T U SHIP Between substrate) and enzymes with high KM values? With low Km values? Make a generalization. Occurs when we know [s] must be equal to KM, Km refers to binding affinity of enzyme 10 Substrate - higher km- tow binding affinity - WoW Km= high binding Affinity [5]= 1.5.6 = 1.75 5. a. What is the Ky value as you can estimate it from Graph 1? S pe =AY-1.75 -0_1.17 13.61...
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the...
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the concentration of the substrate of the reaction ([S]). In Damon's Michaelis-Menten experiment, the highest concentration of substrate used was 500 UM. What do you think will happen to the reaction velocity if higher concentrations of substrate were used? Select one: a. Vo will reach a plateau at higher (S) values O b. Vo will increase exponentially as (S) is increased O c. Vo will...
2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the...
iAt p6, carbonic anhydrase has a keue of 60,000s'. please estimate the ku, if the phf is 13 increased to 7.0. Please justify your answer. Co,+ H20H2CO 14. The two person have the same k after drinking alcohol. Please calculate the substrate earbonie enhydrase concentration in Person 1. Please justify your answer. Person 1 Aldehyde koolS') Km (M) erson 2 dehydrogenase 0.0002 0.006 0.002 15. There is a tyrosine protease, which uses similar mechanism to a cysteine protease. Write down...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
help with these please
The turnover number is defined as the maximum number of substrate molecules that can be converted into product molecules per unit time by an enzyme molecule. The concentration of enzyme active sites is not necessarily equal to the concentration of enzyme molecules, because some enzyme molecules have more than one active site. If the enzyme molecule has one active site, the turnover number is given by turnover number = - -=k2 (Rmax is often written as...
con condition o ste is half its maximal val 9. The Difference between Cellulose and Starch is A Starch doesn't Branch while Cellulose does. Starch contains alpha 16 pycosidic bonds, while Cellulose has beta 1,6 glycosidic bonds. C Starch is made up of carbohydrates and proteins, while cellulose is all carbohydrates Starch is in animals, while Cellulose is in plants. There is no difference between the two 10). An Enzyme catalyzes two different substrates, A and B. The Km for...
DI LUS his tell you about the T U SHIP Between substrate) and enzymes with high KM values? With low Km values? Make a generalization. Occurs when we know [s] must be equal to KM, Km refers to binding affinity of enzyme 10 Substrate - higher km- tow binding affinity - WoW Km= high binding Affinity [5]= 1.5.6 = 1.75 5. a. What is the Ky value as you can estimate it from Graph 1? S pe =AY-1.75 -0_1.17 13.61...
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the concentration of the substrate of the reaction ([S]). In Damon's Michaelis-Menten experiment, the highest concentration of substrate used was 500 UM. What do you think will happen to the reaction velocity if higher concentrations of substrate were used? Select one: a. Vo will reach a plateau at higher (S) values O b. Vo will increase exponentially as (S) is increased O c. Vo will...
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