Question

2. (15 pts) Carbonic anhydrase catalyzes the interconversion between carbon dioxide, water and the dissociated ions of carbonic acid. The following are the initial rate data for this reaction which were obtained in a 1-mL solution that contained 0.0025 gram of carbonic anhydrase (mw -29,100 Da): CO2] (mM) Vo (mM/min) 0.88 1.55 4 2.47 16 3.54 32 4.50 5.21 64 (a) Plot these data in a Lineweaver-Burke plot. Label both axes including proper units. (b) Determine KM, Vmax and the turnover number (keat).

Answer 1

lineweaver-burk plot 1.2 1 y=1.9482x +0.1607 0.8 1/V (min/mM) 0.6 0.4 0.2 0 0.1 0.2 0.3 0.4 0.5 0.6 1/ICO2] (1/mM)

miry mM (ma) Vnmmin) O-88 1-13C O.S .55 O.9S 0. 645 9.u7 0.195 O.40S 16 3.54 O. 289 O.0695 4.S0 39 0.03125 0.a88 5.21 0.0156 0.199 64 gie en data we calculat We plot gaph hdoen ee gut 1.gu8ax t0.1607 Comparing oith Linevoraver Buek E Km VYnar S Vmat et6o7 VmaxG-223 MMin Vmax Km 19482x 6.223 Km +-9482 Vmaz Km=12.123 m catalyet Ginoen mass of O0025 gram 29100 Da 29100 9/mal Volume JmL 1 malority 8.59X1o 29l00 gran/mol 1MLX(IL Jooomt tumoee number (Kead 6.a93 mmmin 8.59163mm VYmax 72.445 min Teachrs SHtre Ans