Question

Review Questions 1) What is your PTC genotype for the C G SNP? 2) This mutation affects the codon sequence resulting in the following change: CCA(Taster)GCA(Nontaster). Does the CG SNP affect what amino acid is encoded by this codon? If so, how? Would you expect this mutation to change the function of the bitter taste receptor? If so, hypothesize how? 3) What is the recognition site for HaelII? 4) Why could we not use the RFLP with HaelII to determine the CT SNP? 5) Could you use the 5' nuclease activity assay to determine the C G polymorphisms? Describe why or why not? 6) Describe (in detail) the similarities and differences in qPCR and semi-quantitative PCR.

Answer 1

1: the gene for the PTC taste receptors TAS2R38, was indentified concept of pharmacogenetics where a SNP genotype is used to predict how does the Haelll enzyme discriminate between the C-G

3:Haelllbis one of many restrictions enzymes discovered since 1970 it was the third Endonuclease to be isolated from the Haemophilus aegytius bacteria, and has a molecular weight of 37126.the enzymes recognition site the place where it cuts DNA moleculesbisbthe GGCC nucleotide sequence

6:RT-PCR is used to amplify the reversed transcription of the DNA code;QPCR measure the amplification 3 RT-PCR is for amplification, while qPCR is quantification 4.QPCR is quantitative in nature, while RT-PCR is not

QPCR differs greatly from RT-PCR, as qPCR IS... of concentration may lead to having "semi quantitative" Inference RT-PCR for amplification, while qPCR is for quantification