Question

PC12 cells produce dopamine and so they may be useful in treating Parkinson's disease (when dopamine is missing). One approach would be to encapsulate PC12 cells inside a spherical capsule (400 pm in outer diameter (OD): 25-um wall thickness) and then implant these capsules inside the brain. The capsules serve to protect the cells from the brain of the recipient. Under one set of circumstances, the cell density in the capsules is initially 1 x 100 cells/mL, and the capsules are incubated in vitro until enough cells grow up to fill the entire capsule.

Answer 1

SOLUTION:

LET THE PC-12 LINE WAS DERIVED FROM A RAT PHEOCHROMOCYTOMA, AN ADRENAL MEDULLARY HUMOR. THE RAT HAVE AN EMBRYONIC ORIGIN FROM THE NEURAL CHEST THAT HAS A MIXTURE OF NEUROBLASTIC CELLS AND EOSINOPHILIC CELLS.

THIS CELL LINE WAS FIRST CULTURED BY GREECE AND TISCHLER IN 1976.

IT WAS DEVELOPED IN PARALLEL TO THE ADRENAL CHROMAFFIN CELL MODEL BECAUSE OF ITS EXTREME VERSATILITY FOR PHARMACOLOGICAL MANIPULATION, EASE OF CULTURE, AND A LARGE AMOUNT OF INFORMATION ON THEIR PROLIFERATION AND DIFFERENTIATION.

THE PC12 CELL LINE HAS BEEN USED TO GET MORE INFORMATION ABOUT DISEASES OF THE BRAIN.

IT HAS BEEN USED IN RESEARCH OF HYPOXIA INDUCES EXOCYTOSIS AND PROLONGED HYPOXIA CAN INDUCE EXCESSIVE EXOCYTOSIS. PC12 CELLS WERE USED TO FIND WHICH PRION PROTEIN FRAGMENTS CAUSED NEURONAL DYSFUNCTION.

PC 12 CELLS A WIDELY USED MODEL NEURONAL CELL LINE, ARE USUALLY CULTURED IN SERUM SUPPLEMENTED MEDIUM.

THANK YOU.......